Elasad Mohammed, Ahmad Adeel, Wang Hantao, Ma Liang, Yu Shuxun, Wei Hengling
Agricultural Research Corporation, Wad Medani P.O. Box 126, Sudan.
State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China.
Plants (Basel). 2020 Oct 19;9(10):1388. doi: 10.3390/plants9101388.
Upland cotton ( L.) is the main natural fiber crop worldwide and is an essential source of seed oil and biofuel products. Many abiotic stresses, such as drought and salinity, constrain cotton production. Thioredoxins (TRXs) are a group of small ubiquitous proteins that are widely distributed among organisms. TRXs play a crucial role in regulating diverse functions during plant growth and development. In the present study, a novel gene was characterized and overexpressed in and silenced in cotton under drought stress. Furthermore, the proline content and enzyme activity levels were measured in transgenic plants and wild-type (Wt) plants under drought and salt stress. The results revealed that the overexpression of enhanced abiotic stress tolerance. When was silenced, cotton plants become more sensitive to drought. Taken together, these findings confirmed that the overexpression of improved drought and salt tolerance in plants. Therefore, the gene can be transformed into cotton plants to obtain transgenic lines for more functional details.
陆地棉是全球主要的天然纤维作物,也是种子油和生物燃料产品的重要来源。许多非生物胁迫,如干旱和盐度,限制了棉花生产。硫氧还蛋白(TRXs)是一类广泛存在于生物体中的小蛋白质。TRXs在植物生长发育过程中调节多种功能方面起着关键作用。在本研究中,一个新基因在干旱胁迫下在棉花中被鉴定、过表达和沉默。此外,还测定了转基因植物和野生型(Wt)植物在干旱和盐胁迫下的脯氨酸含量和酶活性水平。结果表明,该基因的过表达增强了非生物胁迫耐受性。当该基因被沉默时,棉花植株对干旱变得更加敏感。综上所述,这些发现证实了该基因的过表达提高了棉花植株的耐旱性和耐盐性。因此,可以将该基因转化到棉花植株中以获得转基因系,以了解更多功能细节。