Guangxi Key Laboratory of Biology for Mongo, Baise University, Baise, 533000, China.
College of Agriculture and Food Engineering, Baise University, Baise, 533000, China.
BMC Plant Biol. 2024 Mar 23;24(1):208. doi: 10.1186/s12870-024-04908-w.
Mango (Mangifera indica L.) faces escalating challenges from increasing drought stress due to erratic climate patterns, threatening yields, and quality. Understanding mango's drought response mechanisms is pivotal for resilience and food security.
Our RNA-seq analyses unveil 12,752 differentially expressed genes linked to stress signaling, hormone regulation, and osmotic adjustment. Weighted Gene Co-expression Network Analysis identified three essential genes-WRKY transcription factor 3, polyamine oxidase 4, and protein MEI2-like 1-as drought defense components. WRKY3 having a role in stress signaling and defense validates its importance. Polyamine oxidase 4, vital in stress adaptation, enhances drought defense. Protein MEI2-like 1's significance emerges, hinting at novel roles in stress responses. Metabolite profiling illuminated Mango's metabolic responses to drought stress by presenting 990 differentially abundant metabolites, mainly related to amino acids, phenolic acids, and flavonoids, contributing to a deeper understanding of adaptation strategies. The integration between genes and metabolites provided valuable insights by revealing the correlation of WRKY3, polyamine oxidase 4 and MEI2-like 1 with amino acids, D-sphingnosine and 2,5-Dimethyl pyrazine.
This study provides insights into mango's adaptive tactics, guiding future research for fortified crop resilience and sustainable agriculture. Harnessing key genes and metabolites holds promise for innovative strategies enhancing drought tolerance in mango cultivation, contributing to global food security efforts.
由于不稳定的气候模式导致干旱胁迫不断加剧，芒果（Mangifera indica L.）面临着越来越大的挑战，这威胁到了芒果的产量和质量。了解芒果对干旱的响应机制对于提高其韧性和保障粮食安全至关重要。
我们的 RNA-seq 分析揭示了 12752 个与胁迫信号、激素调节和渗透调节相关的差异表达基因。加权基因共表达网络分析确定了三个关键基因——WRKY 转录因子 3、多胺氧化酶 4 和蛋白 MEI2 样 1——作为干旱防御成分。WRKY3 在胁迫信号和防御中起作用，证明了其重要性。多胺氧化酶 4 在应激适应中至关重要，增强了干旱防御能力。蛋白 MEI2 样 1 的重要性凸显出来，暗示其在应激反应中具有新的作用。代谢物分析通过呈现 990 个差异丰度代谢物，主要与氨基酸、酚酸和类黄酮有关，阐明了芒果对干旱胁迫的代谢反应，从而更深入地了解了适应策略。基因和代谢物之间的整合通过揭示 WRKY3、多胺氧化酶 4 和 MEI2 样 1 与氨基酸、D-神经鞘氨醇和 2,5-二甲基吡嗪的相关性提供了有价值的见解。
本研究深入了解了芒果的适应策略，为加强作物韧性和可持续农业的未来研究提供了指导。利用关键基因和代谢物有望为提高芒果抗旱性的创新策略提供思路，为全球粮食安全努力做出贡献。
