Rao Priyanka Singh, Bajaj Rajesh, Mann Bimlesh
Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana 132001 India.
J Food Sci Technol. 2020 Dec;57(12):4562-4575. doi: 10.1007/s13197-020-04495-2. Epub 2020 May 14.
This paper shows the potential of dual enzyme approach on antioxidant activity of casein hydrolysates. Casein was hydrolysed using the proteolytic enzymes alcalase, flavourzyme in isolation and in sequential order. Casein hydrolysates were evaluated for the degree of hydrolysis, antioxidant activity, molecular weight distribution patterns and peptide sequence. Casein hydrolysate produced by the sequential hydrolysis of alcalase and flavourzyme showed higher degree of hydrolysis and antioxidant activity as compared to hydrolysate obtained by individual enzymes. In size exclusion chromatograph of casein hydrolysate S3, peptides with molecular weight of 0.57 kDa share 12% area in total area of chromatogram which was 10 times higher than that of hydrolysate S1 and nearly half of that of hydrolysate S2. On subjecting to HPLC-TOF-ESI separation potential antioxidant peptides were identified. The peptide sequence VLPVPQ along with potential fragments was identified in hydrolysate S1 and S2 and HPHPHLS along with its potential sequence was identified in hydrolysate S1, S2 and S3. Sequential hydrolysis of casein showed better antioxidant activity and peptide profile in less duration as compared to the casein hydrolysate obtained by individual enzyme.
本文展示了双酶法在酪蛋白水解物抗氧化活性方面的潜力。使用蛋白水解酶碱性蛋白酶、风味酶单独及按顺序对酪蛋白进行水解。对酪蛋白水解物的水解度、抗氧化活性、分子量分布模式和肽序列进行了评估。与单独使用酶获得的水解物相比，碱性蛋白酶和风味酶顺序水解产生的酪蛋白水解物显示出更高的水解度和抗氧化活性。在酪蛋白水解物S3的尺寸排阻色谱图中，分子量为0.57 kDa的肽在色谱图总面积中占12%，这比水解物S1高10倍，几乎是水解物S2的一半。通过高效液相色谱-飞行时间-电喷雾电离分离鉴定出潜在的抗氧化肽。在水解物S1和S2中鉴定出肽序列VLPVPQ及其潜在片段，在水解物S1、S2和S3中鉴定出HPHPHLS及其潜在序列。与单独使用酶获得的酪蛋白水解物相比，酪蛋白的顺序水解在更短时间内显示出更好的抗氧化活性和肽谱。
