Siriwat Warin, Ungwiwatkul Sunisa, Unban Kridsada, Laokuldilok Thunnop, Klunklin Warinporn, Tangjaidee Pipat, Potikanond Saranyapin, Kaur Lovedeep, Phongthai Suphat
Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand.
Chemical Industrial Process and Environment Program, Faculty of Science, Energy and Environment, King Mongkut's University of Technology North Bangkok (Rayong Campus), Rayong 21120, Thailand.
Foods. 2023 Oct 18;12(20):3815. doi: 10.3390/foods12203815.
The global plant-based protein demand is rapidly expanding in line with the increase in the world's population. In this study, ultrasonic-assisted extraction (UAE) was applied to extract protein from as an alternative source. Enzymatic hydrolysis was used to modify the protein properties for extended use as a functional ingredient. The successful optimal conditions for protein extraction included a liquid to solid ratio of 30 mL/g, 25 min of extraction time, and a 78% sonication amplitude, providing a higher protein extraction yield than alkaline extraction by about 2.17-fold. The derived protein was rich in essential amino acids, including leucine, valine, and phenylalanine. Protamex and Alcalase were used to prepare protein hydrolysates with different degrees of hydrolysis, producing protein fragments with molecular weights ranging between <10 and 61.5 kDa. Enzymatic hydrolysis caused the secondary structural transformations of proteins from β-sheets and random coils to α-helix and β-turn structures. Moreover, it influenced the protein functional properties, particularly enhancing the protein solubility and emulsifying activity. Partial hydrolysis (DH3%) improved the foaming properties of proteins; meanwhile, an excess hydrolysis degree reduced the emulsifying stability and oil-binding capacity. The produced protein hydrolysates showed potential as anti-cancer peptides on human ovarian cancer cell lines.
随着世界人口的增加，全球对植物性蛋白质的需求正在迅速扩大。在本研究中，采用超声辅助提取（UAE）从[具体来源未给出]中提取蛋白质作为替代来源。使用酶水解来改变蛋白质的性质，以便作为功能性成分进行更广泛的应用。蛋白质提取的成功最佳条件包括液固比为30 mL/g、提取时间为25分钟和超声振幅为78%，其蛋白质提取产率比碱性提取高约2.17倍。所得蛋白质富含必需氨基酸，包括亮氨酸、缬氨酸和苯丙氨酸。使用Protamex和Alcalase制备了不同水解度的蛋白质水解物，产生了分子量在<10至61.5 kDa之间的蛋白质片段。酶水解导致蛋白质的二级结构从β-折叠和无规卷曲转变为α-螺旋和β-转角结构。此外，它还影响了蛋白质的功能特性，特别是提高了蛋白质的溶解度和乳化活性。部分水解（DH3%）改善了蛋白质的发泡性能；同时，过度水解程度降低了乳化稳定性和油结合能力。所制备的蛋白质水解物在人卵巢癌细胞系上显示出作为抗癌肽的潜力。
