新鲜玉米纤维中阿拉伯木聚糖双酶法提取工艺的优化
Optimisation of double-enzymatic extraction of arabinoxylan from fresh corn fibre.
作者信息
Ma Fumin, Li Xiaolei, Yin Jiayu, Ma Lin, Li Dan
机构信息
Key Laboratory of Agroproducts Processing Technology at Jilin Provincial Universities, Education Department of Jilin Provincial Government, College of Food Science and Engineering, Changchun University, Changchun, 130022 Jilin People's Republic of China.
School of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118 People's Republic of China.
出版信息
J Food Sci Technol. 2020 Dec;57(12):4649-4659. doi: 10.1007/s13197-020-04502-6. Epub 2020 May 13.
Enzymatic extraction of arabinoxylans (AXs) is an attractive and environmentally friendly extraction option, in which technical considerations (yield and purity) have been coupled with environmental concerns. Amano HC 90 and Cellulase were combined to evaluate their interactive effects on AX extraction from destarched, deproteinised bran (DSDPB). A response surface methodology was used to obtain the optimal extraction conditions. The experimental data fit well with the predicted values and the model adequately represented the actual relationship among the measured parameters. The extraction yield and AX content in the extract under optimal conditions (double-enzyme dose of 920 U/g, pH of 3.0, extraction temperature of 35.0 °C; extraction time of 6 h; and DSDPB to liquid ratio of 1:30) were 40.73 ± 0.09% and 75.88 ± 0.11%, respectively. The double-enzymatic extraction method of AX from fresh corn fibre was more efficient than the chemical method.
阿拉伯木聚糖（AXs）的酶法提取是一种具有吸引力且环保的提取方法，其中技术考量（产量和纯度）与环境问题相结合。将天野HC 90和纤维素酶组合起来，评估它们对从脱淀粉、脱蛋白麸皮（DSDPB）中提取AX的交互作用。采用响应面法获得最佳提取条件。实验数据与预测值拟合良好，该模型充分代表了测量参数之间的实际关系。在最佳条件下（双酶剂量为920 U/g、pH为3.0、提取温度为35.0℃、提取时间为6小时、DSDPB与液体比例为1:30）提取物中的提取率和AX含量分别为40.73±0.09%和75.88±0.11%。从新鲜玉米纤维中提取AX的双酶法比化学方法更有效。
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