Departament of Biochemistry and Microbiology, Institute of Biosciences, São Paulo State University (UNESP), Avenida 24 A, 1515, ZIP Code 13506-900 Rio Claro, SP, Brazil; Laboratory of Development of Bioprocesses, Bioenergy Research Institute (IPBEN), School of Sciences and Languages, São Paulo State University (UNESP), Avenida Dom Antonio, 2100, ZIP Code 19806-900 Assis, SP, Brazil.
Laboratory of Development of Bioprocesses, Bioenergy Research Institute (IPBEN), School of Sciences and Languages, São Paulo State University (UNESP), Avenida Dom Antonio, 2100, ZIP Code 19806-900 Assis, SP, Brazil.
Bioresour Technol. 2017 Mar;228:164-170. doi: 10.1016/j.biortech.2016.12.097. Epub 2016 Dec 26.
Lignocellulosic material breakdown by hydrolysis is an important step to open new perspectives for bioenergy and special foods production like prebiotic xylooligosaccharides. Improvement of lignocellulose and arabinoxylan alkaline extraction from sugarcane bagasse and enzymatic hydrolysis were performed. Treatments 1 (10% KOH at 70°C), 3 (5% KOH at 121°C) and ZD method (24% KOH at 35°C) showed solid lignocellulose recovery of respectively 75.2%, 74.2% and 73%. A range of 24.8-27% extracted material with high arabinoxylan content (72.1-76.3%) was obtained with these treatments. Treatment 1 and 3 exhibited great KOH reduction in the method reaction, 54.1% and 76.2%, respectively. Likewise, in treatment 3 there was a decrease in ethanol consumption (40.9%) when compared to ZD method. The extracted arabinoxylan showed susceptibility to enzymatic hydrolysis with high solid loading (7%) since Trichoderma reesei xylanases were advantageous for xylose production (54.9%), while Aspergillus fumigatus xylanases achieved better XOS production (27.1%).
木质纤维素材料的水解分解是开辟生物能源和特殊食品生产新途径的重要步骤,如益生元木二糖。从甘蔗渣中进行了木质纤维素和阿拉伯木聚糖的碱性提取以及酶水解的改进。处理 1(70°C 时 10%KOH)、处理 3(121°C 时 5%KOH)和 ZD 方法(35°C 时 24%KOH)分别显示出 75.2%、74.2%和 73%的固体木质纤维素回收率。这些处理方法得到了 24.8-27%的具有高阿拉伯木聚糖含量(72.1-76.3%)的提取物。处理 1 和 3 分别表现出 54.1%和 76.2%的方法反应中 KOH 的大量减少。同样,与 ZD 方法相比,处理 3 中乙醇的消耗减少了 40.9%。提取的阿拉伯木聚糖在高固体负载(7%)下显示出对酶水解的敏感性,因为里氏木霉木聚糖酶有利于木糖的生产(54.9%),而烟曲霉木聚糖酶则更有利于 XOS 的生产(27.1%)。