Quan Fu-Shi, Kong Hyun-Hee, Lee Hae-Ahm, Chu Ki-Back, Moon Eun-Kyung
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, Republic of Korea.
Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
Heliyon. 2020 Oct 12;6(10):e05238. doi: 10.1016/j.heliyon.2020.e05238. eCollection 2020 Oct.
grows intracellularly in free-living amoeba as well as in mammalian macrophages. Until now, the overall gene expression pattern of intracellular in was not fully explained. Intracellular bacteria are capable of not only altering the gene expression of its host, but it can also regulate the expression of its own genes for survival. In this study, differentially expressed genes within during the 24 h intracellular growth period were investigated for comparative analysis. RNA sequencing analysis revealed 3,003 genes from the intracellular . Among them, 115 genes were upregulated and 1,676 genes were downregulated more than 2 fold compared to the free . Gene ontology (GO) analysis revealed the suppression of multiple genes within the intracellular , which were categorized under 'ATP binding' and 'DNA binding' in the molecular function domain. Gene expression of alkylhydroperoxidase, an enzyme involved in virulence and anti-oxidative stress response, was strongly enhanced 24 h post-intracellular growth. Amino acid ABC transporter substrate-binding protein that utilizes energy generation was also highly expressed. Genes associated with alkylhydroperoxidase, glucose pathway, and Dot/Icm type IV secretion system were shown to be differentially expressed. These results contribute to a better understanding of the survival strategies of intracellular within .
它在自由生活的变形虫以及哺乳动物巨噬细胞内生长。到目前为止，其在细胞内的整体基因表达模式尚未得到充分解释。细胞内细菌不仅能够改变宿主的基因表达，还能调节自身基因的表达以实现生存。在本研究中，对其在24小时细胞内生长期间差异表达的基因进行了研究以进行比较分析。RNA测序分析揭示了来自细胞内的3003个基因。其中，与自由状态相比，115个基因上调，1676个基因下调超过2倍。基因本体论（GO）分析揭示了细胞内多个基因的抑制，这些基因在分子功能域中被归类为“ATP结合”和“DNA结合”。参与毒力和抗氧化应激反应的烷基过氧化氢酶的基因表达在细胞内生长24小时后强烈增强。利用能量产生的氨基酸ABC转运体底物结合蛋白也高度表达。与烷基过氧化氢酶、葡萄糖途径和Dot/Icm IV型分泌系统相关的基因显示出差异表达。这些结果有助于更好地理解其在细胞内的生存策略。
