识别蝾螈灯刷染色体上转录单位蛋白的单克隆抗体。
Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes.
作者信息
Roth M B, Gall J G
机构信息
Department of Embryology, Carnegie Institution, Baltimore, Maryland 21210.
出版信息
J Cell Biol. 1987 Sep;105(3):1047-54. doi: 10.1083/jcb.105.3.1047.
Abstract
We prepared hybridoma cell lines from mice injected with newt germinal vesicle proteins. We tested culture supernates from these cell lines for antibodies that bound to specific morphological structures in lampbrush chromosome preparations (nucleoli, loops, chromomeres, etc.). Four mAbs that recognize antigens on the lateral transcription loops are described here. We suggest that these antigens are proteins associated with nascent RNA transcripts, although they are not among the 30-40-kD "core" heterogeneous nuclear ribonucleoproteins.
摘要
我们用注射了蝾螈生发泡蛋白的小鼠制备了杂交瘤细胞系。我们检测了这些细胞系的培养上清液，以寻找能与灯刷染色体标本中的特定形态结构（核仁、环、染色粒等）结合的抗体。本文描述了四种识别侧转录环上抗原的单克隆抗体。我们认为这些抗原是与新生RNA转录本相关的蛋白质，尽管它们并不属于30 - 40kD的“核心”不均一核核糖核蛋白。
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