Fuller K, Chambers T J
Department of Histopathology, St George's Hospital Medical School, London, United Kingdom.
J Cell Physiol. 1987 Sep;132(3):441-52. doi: 10.1002/jcp.1041320306.
The primary and specific function of the osteoclast is the resorption of bone. We have applied this criterion, and a monoclonal antibody that binds specifically to osteoclasts, to cultures of tissues that may contain osteoclastic precursors. Bone marrow and spleen cells were incubated for up to 4 weeks in the presence or absence of parathyroid hormone, interleukin 1, or 1,25(OH)2 vitamin D3, on plastic coverslips or slices of devitalised bone. Osteoclasts (as judged by the presence of resorption cavities and the appearance of monoclonal antibody-positive cells) did not develop in cultures incubated without added hormones, nor in cultures containing parathyroid hormone or interleukin 1, but were regularly observed when bone marrow cells were incubated with 1,25(OH)2 vitamin D3. Although multinucleate giant cells were common after incubation, especially in the presence 1,25(OH)2 vitamin D3, monoclonal antibody bound not to these cells but to a minor and distinctive population of mononuclear cells and cells of low multinuclearity. We found no excavations and no monoclonal antibody-positive cells after incubation of peritoneal macrophages with 1,25(OH)2D3. These results provide direct evidence of osteoclastic function arising in cultures of haemopoietic tissues.
破骨细胞的主要特定功能是骨吸收。我们已将此标准以及一种能特异性结合破骨细胞的单克隆抗体应用于可能含有破骨细胞前体的组织培养。将骨髓和脾细胞在有无甲状旁腺激素、白细胞介素1或1,25(OH)₂维生素D₃存在的情况下，在塑料盖玻片或失活骨切片上培养长达4周。在未添加激素的培养物中，以及在含有甲状旁腺激素或白细胞介素1的培养物中，均未形成破骨细胞（根据吸收腔的存在以及单克隆抗体阳性细胞的出现来判断），但当骨髓细胞与1,25(OH)₂维生素D₃一起培养时，经常可观察到破骨细胞。尽管培养后多核巨细胞很常见，尤其是在有1,25(OH)₂维生素D₃存在的情况下，但单克隆抗体并非与这些细胞结合，而是与一小部分独特的单核细胞和低多核性细胞结合。在用1,25(OH)₂D₃培养腹膜巨噬细胞后，我们未发现侵蚀现象，也未发现单克隆抗体阳性细胞。这些结果为造血组织培养中出现破骨细胞功能提供了直接证据。
