Horton M A, Rimmer E F, Chambers T J
Department of Haematology, St. Bartholomew's Hospital, London, UK.
J Bone Miner Res. 1986 Feb;1(1):5-14. doi: 10.1002/jbmr.5650010104.
A method for the long-term culture of rabbit newborn bone marrow has been developed. It is characterized by the rapid appearance of an adherent, adipocyte-containing stromal layer, proliferation of mature myeloid cells, and the formation of numerous, large multinucleate giant cells. By the combined use of morphological, immunological, and functional criteria these giant cells have been characterized as macrophage polykaryons and not osteoclastic giant cells. We conclude that long-term bone marrow culture in the rabbit favors the proliferation, maturation, and fusion of macrophage, but not osteoclast, precursors--new experimental models will have to be developed to enable the developmental biology of osteoclasts to be studied in the rabbit.
已开发出一种用于兔新生骨髓长期培养的方法。其特点是迅速出现一层贴壁的、含脂肪细胞的基质层，成熟髓样细胞增殖，并形成大量大型多核巨细胞。通过综合运用形态学、免疫学和功能标准，这些巨细胞已被鉴定为巨噬细胞多核体而非破骨巨细胞。我们得出结论，兔骨髓长期培养有利于巨噬细胞而非破骨细胞前体的增殖、成熟和融合——必须开发新的实验模型，以便能够在兔体内研究破骨细胞的发育生物学。
