Li Wen-Jing, Chen Hui-Zhen, Liu Cong-Na, Zahng Zhi-Yong
Department of Oral Medicine, the Second Hospital of Hebei Medical University. Shijiazhuang 050000, Hebei Province，China. E-mail:
Shanghai Kou Qiang Yi Xue. 2020 Aug;29(4):365-369.
To investigate the role of P2X7 receptor in osteogenic differentiation of human periodontal ligament stem cells.
Human periodontal ligament stem cells obtained from primary culture were divided into 4 groups: control group, adenosine triphosphate group, osteogenic induction group, adenosine triphosphate + osteogenic induction group. The differences of RUNX2, OCN gene expression and P2X7 receptor mRNA expression between the four groups were compared. Statistical analysis was performed with SPSS 16.0 software package.
One week after osteogenic formation and two weeks after osteogenic formation, the expression of RUNX2 and OCN mRNA in the adenosine triphosphate + osteogenic induction group was significantly higher than that in the osteogenic induction group (P<0.05). The expression of RUNX2 and OCN mRNA in the 1 week after adenosine triphosphate + osteogenic induction fluid was significantly higher than that 2 weeks after osteogenic formation, and the difference was statistically significant (P<0.05). The expression of P2X7 receptor mRNA in the adenosine triphosphate group and the adenosine triphosphate + osteogenic induction group was significantly higher than that in the control group and the osteogenic induction group 1 week after osteogenesis and 2 weeks after osteogenesis (P<0.05). The expression of P2X7 receptor mRNA in the adenosine triphosphate group was significantly higher than that in the adenosine triphosphate + osteogenic induction group 2 weeks after osteogenesis(P<0.05). The expression of P2X7 receptor mRNA was significantly higher than that of osteogenic induction 1 week after adenosine triphosphate composition(P<0.05).
P2X7 receptor can significantly improve the osteogenesis of periodontal ligament stem cells, and adenosine triphosphate can activate the expression of P2X7 receptor.
探讨P2X7受体在人牙周膜干细胞成骨分化中的作用。
将原代培养获得的人牙周膜干细胞分为4组：对照组、三磷酸腺苷组、成骨诱导组、三磷酸腺苷+成骨诱导组。比较4组间RUNX2、OCN基因表达及P2X7受体mRNA表达的差异。采用SPSS 16.0软件包进行统计学分析。
成骨形成1周后及成骨形成2周后，三磷酸腺苷+成骨诱导组RUNX2和OCN mRNA的表达明显高于成骨诱导组（P<0.05）。三磷酸腺苷+成骨诱导液作用1周后RUNX2和OCN mRNA的表达明显高于成骨形成2周后，差异有统计学意义（P<0.05）。成骨1周后及成骨2周后，三磷酸腺苷组和三磷酸腺苷+成骨诱导组P2X7受体mRNA的表达明显高于对照组和成骨诱导组（P<0.05）。成骨2周后，三磷酸腺苷组P2X7受体mRNA的表达明显高于三磷酸腺苷+成骨诱导组（P<0.05）。三磷酸腺苷组成后1周P2X7受体mRNA的表达明显高于成骨诱导组（P<0.05）。
P2X7受体可显著促进牙周膜干细胞的成骨作用，三磷酸腺苷可激活P2X7受体的表达。
