GEROM Groupe d'Etude Remodelage Osseux et bioMatériaux, LHEA, IRIS-IBS Institut de Biologie en Santé, Université d'Angers, CHU d'Angers, Angers Cedex, France.
Service de chirurgie maxillo-faciale et stomatologie, CHU d'Angers, Angers Cedex, France.
J Biomed Mater Res B Appl Biomater. 2021 Jun;109(6):780-788. doi: 10.1002/jbm.b.34743. Epub 2020 Oct 21.
The use of resorbable devices for osteosynthesis has become a subject of interest. Nacre has been proposed as a resorbable and osteoconductive material favoring bone apposition without triggering an inflammatory reaction. We compared the in vivo osseointegration and erosion of nacre screws in an animal model with titanium screws. Implantation of similar nacre and titanium screws was performed in the femoral condyles of adult rats. Animals (n = 41) were randomized in four groups sacrificed at day one, 1, 6, and 12 months. Microcomputed tomography (microCT) allowed 3D morphometry of erosion of nacre. Osseointegration was measured as the volume of trabecular bone bone volume/tissue volume (BV/TV) in a standardized volume of interest around each screw. Undecalcified bone histology was also done. Gross examination revealed a similar clinical osseointegration for titanium and nacre screws. A progressive erosion of nacre screws, but no erosion of titanium screws, was observed in microCT. The volume of nacre screws progressively decreased over time whereas no modification occurred for titanium. For titanium screws, BV/TV remained stable throughout the study. For nacre screws, the BV/TV decrease was not statistically different. A significant difference was found between nacre and titanium screws at 6 months but not at 12 months. The screw heads, outside the bone shaft, were not eroded even after 12 months. Erosion of nacre occurred during the entire study period, only within the bone shaft in direct contact with bone marrow. Bone apposition was observed on nacre surfaces without signs of erosion. Nacre is a promising biomaterial in maxillofacial surgery.
可吸收装置在骨折内固定中的应用已成为研究热点。珍珠母已被提议作为一种可吸收且具有骨诱导性的材料，有利于骨沉积而不引发炎症反应。我们在动物模型中比较了珍珠母螺钉和钛螺钉的体内骨整合和侵蚀情况。在成年大鼠的股骨髁中植入了类似的珍珠母和钛螺钉。将动物（n = 41）随机分为 4 组，在第 1、1、6 和 12 个月时处死。微计算机断层扫描（microCT）允许对珍珠母的 3D 形态进行侵蚀测量。将螺钉周围标准化感兴趣体积内的骨小梁骨体积/组织体积（BV/TV）作为骨整合的测量值。还进行了未脱钙骨组织学检查。大体检查显示钛和珍珠母螺钉的临床骨整合相似。在 microCT 中观察到珍珠母螺钉逐渐侵蚀，但钛螺钉没有侵蚀。随着时间的推移，珍珠母螺钉的体积逐渐减少，而钛螺钉没有变化。对于钛螺钉，BV/TV 在整个研究过程中保持稳定。对于珍珠母螺钉，BV/TV 的减少没有统计学差异。在 6 个月时，珍珠母和钛螺钉之间存在显著差异，但在 12 个月时没有差异。螺钉头部（在骨轴外）即使在 12 个月后也没有被侵蚀。珍珠母的侵蚀发生在整个研究期间，仅在与骨髓直接接触的骨轴内。在珍珠母表面观察到骨沉积，没有侵蚀迹象。珍珠母是颌面外科中一种很有前途的生物材料。
