Tian Yang, Jiang Wen-Li, Wang Wen-Xin, Peng Jie, Li Xue-Meng, Li Yongfei, Li Chun-Yan
Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, PR China.
Analyst. 2021 Jan 4;146(1):118-123. doi: 10.1039/d0an01719a.
As a kind of toxic gas, carbon monoxide (CO) can hinder uptake of oxygen in humans. However, more and more studies have shown that CO is an important gaseous messenger in the body and playing an indispensable role in intracellular signaling pathways. So, it is necessary to develop an analytical method to study CO in living organisms. Although there are many CO-responsive probes, most of them have the disadvantages of a small Stokes shift or short emission wavelength. In order to address the above issue, a novel probe (FDX-CO) with a large Stokes shift (190 nm) and long emission wavelength (770 nm) was firstly synthesized to detect CO. The probe shows high sensitivity and superior selectivity toward CO. Moreover, the probe was successfully used for visualizing exogenous and endogenous CO in cells by fluorescence imaging, 3D quantification analysis and flow cytometric analysis. More importantly, FDX-CO could excellently detect CO in mice, which suggests that this probe has the potential ability to image CO in vivo. This probe can be viewed as a useful tool in the research of CO.
作为一种有毒气体，一氧化碳（CO）会阻碍人体对氧气的吸收。然而，越来越多的研究表明，CO是体内一种重要的气体信使，在细胞内信号通路中发挥着不可或缺的作用。因此，有必要开发一种分析方法来研究生物体内的CO。尽管有许多CO响应探针，但它们大多存在斯托克斯位移小或发射波长短的缺点。为了解决上述问题，首先合成了一种具有大斯托克斯位移（190 nm）和长发射波长（770 nm）的新型探针（FDX-CO）来检测CO。该探针对CO表现出高灵敏度和优异的选择性。此外，通过荧光成像、三维定量分析和流式细胞术分析，该探针成功用于可视化细胞中外源性和内源性CO。更重要的是，FDX-CO能够出色地检测小鼠体内的CO，这表明该探针具有在体内对CO进行成像的潜在能力。该探针可被视为CO研究中的一种有用工具。
