Tang Junshuai, Zhang Pingzhu, Li Zimeng, Zhang Yuehua, Chen Hua, Li Xiaoliu, Wei Chao
College of Chemistry and Materials Science, Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Ministry of Education), Hebei University, Baoding 071002, PR China.
College of Chemistry and Materials Science, Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Ministry of Education), Hebei University, Baoding 071002, PR China.
Bioorg Chem. 2023 Jun;135:106489. doi: 10.1016/j.bioorg.2023.106489. Epub 2023 Mar 21.
Carbon monoxide (CO) is an important gas signaling molecule and has been widely involved in regulating important life processes. Effective monitoring of CO in living systems is critical. Combined with the accuracy of ratio detection and the advantages of two-photon imaging, a simple ratiometric two-photon fluorescent probe RTFP was rationally designed and synthesized using 7-(diethylamino)-4-hydroxycoumarin as a two-photon fluorophore and allyl carbonate as the reactive unit. Probe RTFP exhibited excellent selectivity and sensitivity towards CO, and was successfully applied to image endogenous CO in living cells and zebrafish.
一氧化碳（CO）是一种重要的气体信号分子，广泛参与调节重要的生命过程。对生物系统中的CO进行有效监测至关重要。结合比率检测的准确性和双光子成像的优势，以7-（二乙氨基）-4-羟基香豆素作为双光子荧光团、碳酸烯丙酯作为反应单元，合理设计并合成了一种简单的比率型双光子荧光探针RTFP。探针RTFP对CO表现出优异的选择性和灵敏度，并成功应用于活细胞和斑马鱼体内内源性CO的成像。
