Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.
Int Endod J. 2021 Mar;54(3):399-412. doi: 10.1111/iej.13435. Epub 2020 Nov 18.
To determine whether irisin, a newly discovered myokine that links exercise-induced and metabolic homeostasis, is able to promote odontogenic differentiation and angiogenesis in human dental pulp cells (HDPCs).
Cell viability in the presence of irisin was measured. Real-time PCR and Western blot analysis were performed to evaluate the expression levels of irisin, odontogenic and angiogenic markers. The involvement of mitogen-activated protein kinase (MAPK) and the protein kinase B (Akt) signalling pathway was evaluated by Western blot. To evaluate mineralization nodule formation, alkaline phosphatase (ALP) staining and alizarin red S staining were performed. Scratch wound assays were performed to evaluate the effects of irisin on cell migration. The data were analysed using one-way analysis of variance (anova) followed by Tukey post hoc test and Student's t-test. Statistical significance was considered at P < 0.05.
Irisin significantly promoted odontogenic differentiation as evidenced by formation of mineralized nodules, induction of ALP activity and upregulation of odontogenic and angiogenic markers (P < 0.05). Scratch wound assays revealed that irisin significantly increased migration of HDPCs (P < 0.05). Phosphorylation of both MAPK and Akt was increased by irisin. MAPK and Akt inhibitors inhibited mineralization, cell migration and the increased expression of odontogenic and angiogenic markers.
Irisin promoted odontogenic differentiation and mineralization and has the potential for angiogenesis through activation of the MAPK and Akt signalling pathways in HDPCs.
确定一种新发现的肌因子鸢尾素是否能够促进人牙髓细胞（HDPCs）的牙源性分化和血管生成，这种肌因子可以将运动引起的代谢和内稳态联系起来。
在存在鸢尾素的情况下测量细胞活力。通过实时 PCR 和 Western blot 分析来评估鸢尾素、牙源性和血管生成标记物的表达水平。通过 Western blot 评估丝裂原活化蛋白激酶（MAPK）和蛋白激酶 B（Akt）信号通路的参与情况。通过碱性磷酸酶（ALP）染色和茜素红 S 染色来评估矿化结节形成。通过划痕实验来评估鸢尾素对细胞迁移的影响。使用单因素方差分析（anova）和 Tukey 事后检验以及学生 t 检验来分析数据。P 值小于 0.05 时认为具有统计学意义。
鸢尾素显著促进牙源性分化，表现为矿化结节形成、ALP 活性诱导以及牙源性和血管生成标记物的上调（P ＜ 0.05）。划痕实验表明，鸢尾素显著增加了 HDPCs 的迁移（P ＜ 0.05）。鸢尾素增加了 MAPK 和 Akt 的磷酸化。MAPK 和 Akt 抑制剂抑制了矿化、细胞迁移以及牙源性和血管生成标记物的表达增加。
鸢尾素通过激活 HDPCs 中的 MAPK 和 Akt 信号通路，促进牙源性分化和矿化，并具有促进血管生成的潜力。
