Department of Clinical Laboratory Medicine, and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, China.
Eur Rev Med Pharmacol Sci. 2020 Oct;24(19):9924-9931. doi: 10.26355/eurrev_202010_23203.
The aim of the study was to illustrate the function of circRNA ZFR in aggravating the development of hepatocellular carcinoma (HCC) by upregulating MAP2K1.
CircRNA ZFR levels in 62 paired HCC and paracancerous species were detected. The influence of circRNA ZFR on clinical data of HCC patients was analyzed. After the overexpression or knockdown of circRNA ZFR, changes in viability and clonality of Bel-7402 and Hep3B cells were assessed, respectively. The involvement of circRNA ZFR/MAP2K1 axis in the development of HCC was explored through Luciferase assay and rescue experiments.
CircRNA ZFR was highly expressed in HCC species than the paracancerous ones. Higher level of circRNA ZFR predicted more advanced tumor grading of HCC. The knockdown of circRNA ZFR attenuated the proliferative ability of HCC cells, while the overexpression of circRNA ZFR obtained opposite results. MAP2K1 level was positively correlated to that of circRNA ZFR. Luciferase assay uncovered that circRNA ZFR can be targeted by MAP2K1 through specific binding sites. In addition, the overexpression of MAP2K1 could reverse the influence of silenced circRNA ZFR on proliferative ability of HCC cells.
CircRNA ZFR is upregulated in HCC and closely linked to tumor grading. It promotes proliferative ability in HCC by upregulating MAP2K1.
本研究旨在通过上调 MAP2K1 来阐明 circRNA ZFR 在加剧肝细胞癌（HCC）发展中的作用。
检测了 62 对 HCC 和癌旁组织中 circRNA ZFR 的水平。分析了 circRNA ZFR 对 HCC 患者临床数据的影响。在过表达或敲低 circRNA ZFR 后，分别评估 Bel-7402 和 Hep3B 细胞活力和克隆性的变化。通过荧光素酶测定和挽救实验探讨 circRNA ZFR/MAP2K1 轴在 HCC 发展中的作用。
circRNA ZFR 在 HCC 组织中表达高于癌旁组织。circRNA ZFR 水平较高提示 HCC 的肿瘤分级更先进。circRNA ZFR 的敲低减弱了 HCC 细胞的增殖能力，而 circRNA ZFR 的过表达则获得了相反的结果。MAP2K1 水平与 circRNA ZFR 水平呈正相关。荧光素酶测定揭示 circRNA ZFR 可以通过特定的结合位点被 MAP2K1 靶向。此外，MAP2K1 的过表达可以逆转沉默 circRNA ZFR 对 HCC 细胞增殖能力的影响。
circRNA ZFR 在 HCC 中上调，与肿瘤分级密切相关。它通过上调 MAP2K1 促进 HCC 的增殖能力。
