Newell D G
Experimental Pathology Laboratory, PHLS Centre for Applied Microbiology and Research, Salisbury, UK.
J Gen Microbiol. 1987 Jan;133(1):163-70. doi: 10.1099/00221287-133-1-163.
The outer membrane and surface exposed proteins of four strains of the gastric Campylobacter-like organism Campylobacter pyloridis were identified by SDS-PAGE of Sarkosyl-insoluble membranous material and 125I-surface-labelled whole bacteria. Although constant outer membrane proteins (molecular mass 61, 54 and 31 kDa) were observed in these strains, several variable 125I-labelled surface proteins were detected. C. pyloridis does not appear to express a single surface-exposed major outer membrane protein like that of C. jejuni and C. coli. Putative flagella proteins were identified from isolated flagella and acid-extractable surface material and by immunoblotting with anti-flagella antibodies. Several major protein antigens were observed by immunoblotting with anti-C. pyloridis antisera. At least two of these antigens cross-reacted with anti-C. jejuni antiserum. This cross-reaction appears to be caused primarily by flagellar antigens. However, one major protein antigen (61 kDa) was not cross-reactive with C. jejuni and may, therefore, be useful in serological tests for the specific diagnosis of C. pyloridis infections.
通过对十二烷基肌氨酸钠不溶性膜材料进行SDS-PAGE以及对125I表面标记的全菌进行分析，鉴定了四株胃弯曲菌样生物体幽门弯曲菌的外膜和表面暴露蛋白。尽管在这些菌株中观察到了恒定的外膜蛋白（分子量分别为61、54和31 kDa），但也检测到了几种可变的125I标记表面蛋白。幽门弯曲菌似乎不像空肠弯曲菌和结肠弯曲菌那样表达单一的表面暴露主要外膜蛋白。从分离的鞭毛和酸可提取表面物质中，并通过用抗鞭毛抗体进行免疫印迹，鉴定了假定的鞭毛蛋白。用抗幽门弯曲菌抗血清进行免疫印迹观察到几种主要的蛋白质抗原。其中至少两种抗原与抗空肠弯曲菌抗血清发生交叉反应。这种交叉反应似乎主要是由鞭毛抗原引起的。然而，但一种主要蛋白质抗原（61 kDa）与空肠弯曲菌无交叉反应，因此可能有助于幽门弯曲菌感染特异性诊断的血清学检测。
