Wenman W M, Chai J, Louie T J, Goudreau C, Lior H, Newell D G, Pearson A D, Taylor D E
J Clin Microbiol. 1985 Jan;21(1):108-12. doi: 10.1128/jcm.21.1.108-112.1985.
Outer membrane proteins of Campylobacter jejuni and other campylobacter species were analyzed for their antigenic potentials by immunoblotting. Polypeptides were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred electrophoretically, and reacted with rabbit antisera to C. jejuni. Each Campylobacter species analyzed demonstrated a unique outer membrane protein antigenic profile; interspecies antigen sharing was observed to be compatible with the degree of DNA relatedness between the species. The most highly conserved outer membrane protein antigen was the flagellum (molecular weight, 62,000). An aflagellate mutant was found to be untypable with the heat-labile system, in contrast to its parental isolate. The immunogenic potentials of C. jejuni proteins were examined by immunoblot analysis of sera from infected humans. Sera of convalescent patients, reacted with their homologous C. jejuni isolates, recognized a variety of campylobacter proteins. The most consistent immunogen in human infection was the flagellar protein. Patient sera assayed by the immunoblot technique were easily distinguished from control sera, which did not recognize specific campylobacter antigens. These findings suggest that the campylobacter flagellar protein is an essential determinant of the heat-labile antigen typing scheme and is the dominant immunogen recognized during C. jejuni infections in humans.
通过免疫印迹法分析空肠弯曲菌和其他弯曲菌属的外膜蛋白的抗原潜力。多肽通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离，电泳转移后与抗空肠弯曲菌兔抗血清反应。所分析的每个弯曲菌属都显示出独特的外膜蛋白抗原谱；观察到种间抗原共享与各物种之间的DNA相关性程度相符。最保守的外膜蛋白抗原是鞭毛（分子量为62,000）。与亲本菌株相比，发现一个无鞭毛突变体无法用不耐热系统分型。通过对感染人类血清的免疫印迹分析来检测空肠弯曲菌蛋白的免疫原性潜力。恢复期患者的血清与同源的空肠弯曲菌菌株反应，识别出多种弯曲菌蛋白。人类感染中最一致的免疫原是鞭毛蛋白。通过免疫印迹技术检测的患者血清很容易与不识别特定弯曲菌抗原的对照血清区分开来。这些发现表明，弯曲菌鞭毛蛋白是不耐热抗原分型方案的重要决定因素，并且是人类空肠弯曲菌感染期间识别的主要免疫原。
