猪体内缺失 MGF-360-1L 基因的重组非洲猪瘟病毒的评估。

Evaluation in Swine of a Recombinant African Swine Fever Virus Lacking the MGF-360-1L Gene.

机构信息

Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA.

Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, USA.

出版信息

Viruses. 2020 Oct 20;12(10):1193. doi: 10.3390/v12101193.

The African swine fever (ASF) pandemic is currently affecting pigs throughout Eurasia, resulting in significant swine production losses. The causative agent, ASF virus (ASFV), is a large, structurally complex virus with a genome encoding more than 160 genes. The function of most of those genes remains unknown. Here, we presented the previously uncharacterized ASFV gene MGF360-1L, the first gene in the genome. The kinetic studies of virus RNA transcription demonstrated that the MGF360-1L gene was transcribed as a late virus protein. The essentiality of MGF360-1L to virus replication was evaluated by developing a recombinant ASFV lacking the gene (ASFV-G-ΔMGF360-1L). In primary swine macrophage cell cultures, ASFV-G-ΔMGF360-1L showed similar replication kinetics as the parental highly virulent field isolate Georgia2007 (ASFV-G). Domestic pigs experimentally infected with ASFV-G-ΔMGF360-1L presented with a clinical disease indistinguishable from that caused by ASFV-G, demonstrating that MGF360-1L was not involved in virulence in swine, the natural host of ASFV.

非洲猪瘟（ASF）疫情目前正在欧亚大陆各地的猪群中流行，导致了严重的生猪生产损失。该病的病原体是非洲猪瘟病毒（ASFV），它是一种结构复杂的大型病毒，基因组编码超过 160 个基因。这些基因的大多数功能仍然未知。在这里，我们介绍了以前未被描述的 ASFV 基因 MGF360-1L，它是基因组中的第一个基因。病毒 RNA 转录的动力学研究表明，MGF360-1L 基因作为晚期病毒蛋白转录。通过开发缺失该基因的重组 ASFV（ASFV-G-ΔMGF360-1L）来评估 MGF360-1L 对病毒复制的必要性。在原代猪巨噬细胞培养物中，ASFV-G-ΔMGF360-1L 的复制动力学与亲本高致病性田间分离株格鲁吉亚 2007（ASFV-G）相似。用 ASFV-G-ΔMGF360-1L 实验感染的家猪表现出与 ASFV-G 引起的临床疾病无法区分的症状，表明 MGF360-1L 在家猪（ASFV 的自然宿主）中不参与毒力。