James M A, Montenegro-James S, Fajfar-Whetstone C, Montealegre F, Erickson J, Ristic M
Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.
J Protozool. 1987 Aug;34(3):328-32. doi: 10.1111/j.1550-7408.1987.tb03184.x.
Antigenic similarities between Plasmodium and Babesia parasites of the phylum Apicomplexa have been previously demonstrated primarily by the serological cross reactivity observed in the indirect fluorescent antibody (IFA) test. We have now studied the antigenic relationship between the human malaria parasite, Plasmodium falciparum, and the hemoparasitic agent of cattle, Babesia bovis, using rabbit monospecific antibodies produced against individual culture-derived P. falciparum polypeptides and bovine polyspecific antibodies to B. bovis exoantigens. These respective antibodies were found to be distinctly cross reactive in the IFA test using infected erythrocytes (squirrel monkey--P. falciparum; bovine--B. bovis) as antigen substrates. Immunofluorescence was shown to be highly specific for parasite surfaces. Additionally, the degree of reactivity with soluble exoantigens contained in Plasmodium and Babesia culture supernatants was monitored by a two-site enzyme immunoassay employing the cross-reactive antibodies. Further evidence for antigenic cross reactivity between P. falciparum and B. bovis parasites was shown with the in vitro inhibition assay. Antibodies to P. falciparum and B. bovis were found to be highly inhibitory for the in vitro growth of P. falciparum in human erythrocytes.
之前主要通过间接荧光抗体（IFA）试验中观察到的血清学交叉反应，证明了顶复门的疟原虫和巴贝斯虫寄生虫之间的抗原相似性。我们现在使用针对单个培养来源的恶性疟原虫多肽产生的兔单特异性抗体，以及针对牛巴贝斯虫外抗原的牛多特异性抗体，研究了人类疟原虫恶性疟原虫与牛血液寄生虫牛巴贝斯虫之间的抗原关系。在使用感染红细胞（松鼠猴——恶性疟原虫；牛——牛巴贝斯虫）作为抗原底物的IFA试验中，发现这些各自的抗体具有明显的交叉反应性。免疫荧光显示对寄生虫表面具有高度特异性。此外，采用交叉反应抗体的双位点酶免疫测定法监测了与疟原虫和巴贝斯虫培养上清液中可溶性外抗原的反应程度。体外抑制试验进一步证明了恶性疟原虫和牛巴贝斯虫寄生虫之间的抗原交叉反应性。发现针对恶性疟原虫和牛巴贝斯虫的抗体对恶性疟原虫在人红细胞中的体外生长具有高度抑制作用。
