Hussain Malik Fiaz, Naeem Ashiq Muhammad, Gulsher Muhammd, Akbar Atif, Iqbal Furhan
Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan, Pakistan.
Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.
Biomarkers. 2020 Dec;25(8):719-724. doi: 10.1080/1354750X.2020.1841829. Epub 2020 Nov 3.
This study was designed to report the biological effect of nickel oxide nanoparticles (NiO NPs) in albino mice.
Five weeks old albino mice of both sex were intraperitoneally injected either with 20 mg (low dose) or 50 mg/mL saline/kg body weight (high dose) of NiO NPs for 14 days. Saline-treated controls were maintained in parallel. Complete blood count, selected serum biochemical parameters and oxidative stress biomarkers from vital organs were determined in all subjects.
Male mice treated with NiO NPS had increased blood urea nitrogen, elevated superoxide dismutase (SOD) in liver elevated MDA in liver, kidney and heart and reduced catalase activity in heart and kidney. Female mice treated with NiO NPs had significantly reduced serum albumin and total proteins, SOD in lungs and elevated MDA in liver.
We are reporting that intraperitoneal injections of NiO NPs for 14 days drastically affect blood serum parameters and oxidative stress biomarkers from vital organs of albino mice.
Toxic effects of NiO NPs were dose and sex dependent and they were more pronounced at higher dose and in male mice.
本研究旨在报告氧化镍纳米颗粒（NiO NPs）对白化病小鼠的生物学效应。
对5周龄的雌雄白化病小鼠腹腔注射20毫克（低剂量）或50毫克/毫升生理盐水/千克体重（高剂量）的NiO NPs，持续14天。同时设置生理盐水处理的对照组。测定所有实验对象的全血细胞计数、选定的血清生化参数以及重要器官的氧化应激生物标志物。
用NiO NPs处理的雄性小鼠血尿素氮升高，肝脏中超氧化物歧化酶（SOD）升高，肝脏、肾脏和心脏中丙二醛（MDA）升高，心脏和肾脏中的过氧化氢酶活性降低。用NiO NPs处理的雌性小鼠血清白蛋白和总蛋白显著降低，肺中SOD降低，肝脏中MDA升高。
我们报告腹腔注射NiO NPs 14天会显著影响白化病小鼠重要器官的血清参数和氧化应激生物标志物。
NiO NPs的毒性作用具有剂量和性别依赖性，在高剂量时以及雄性小鼠中更为明显。
