Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057, Braga, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal; Biology Department - University of Minho, School of Sciences (DB-ECUM), Campus de Gualtar, 4710-057, Braga, Portugal.
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057, Braga, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.
Free Radic Biol Med. 2021 Jan;162:202-215. doi: 10.1016/j.freeradbiomed.2020.10.018. Epub 2020 Oct 20.
Hyptis suaveolens (HS), Hyptis pectinata (HP) and Hyptis marrubioides (HM) are plants used in folk medicine for treatment of several diseases. Here, we tested the in vivo antioxidant and neuroprotective potential of methanolic extracts from these plants, containing several rosmarinic acid derivatives and isoquercetin. In C. elegans, HS, HP and HM leaf extracts enhanced the antioxidant responses through the induction of specific antioxidant enzymes and demonstrated neurotherapeutic potential in transgenic models of genetically determined human neurodegenerative diseases - Frontotemporal dementia with parkinsonism linked to chromosome 17 and Machado-Joseph disease. Chronic treatment of disease models with HS, HP and HM leaf extracts improved the animals' motor function and increased their tolerance to an oxidative insult. The restorative effect of HM extract in motor performance of both disease models required the presence of glutathione reductase (gsr-1), an enzyme that assures the glutathione redox cycle, highlighting the role of this pathway and unveiling a common candidate therapeutic target for these diseases. Our findings strengthen the relevance of plant-derived bioactive compound discovery for neurodegenerative disorders that remain without effective treatment.
紫麻（Hyptis suaveolens，HS）、麻叶荨麻（Hyptis pectinata，HP）和马鲁比紫麻（Hyptis marrubioides，HM）是民间医学中用于治疗多种疾病的植物。在这里，我们测试了这些植物的甲醇提取物的体内抗氧化和神经保护潜力，其中含有几种迷迭香酸衍生物和异槲皮素。在秀丽隐杆线虫中，HS、HP 和 HM 的叶片提取物通过诱导特定的抗氧化酶来增强抗氧化反应，并在由染色体 17 相关的额颞叶痴呆和 Machado-Joseph 病引起的人类遗传性神经退行性疾病的转基因模型中显示出神经治疗潜力。用 HS、HP 和 HM 的叶片提取物对疾病模型进行慢性治疗可改善动物的运动功能，并提高其对氧化应激的耐受性。HM 提取物对两种疾病模型的运动性能的恢复作用需要谷胱甘肽还原酶（gsr-1）的存在，谷胱甘肽还原酶是一种确保谷胱甘肽氧化还原循环的酶，突出了该途径的作用，并揭示了这些疾病的共同候选治疗靶点。我们的发现为神经退行性疾病的植物源性生物活性化合物的发现提供了重要支持，这些疾病仍然缺乏有效的治疗方法。
