Vilasboas-Campos Daniela, Costa Marta Daniela, Teixeira-Castro Andreia, Rios Rejaine, Silva Fabiano Guimarães, Aierken Aili, Zhang Xiaoying, Bessa Carlos, Dias Alberto C P, Maciel Patrícia
School of Medicine, Life and Health Sciences Research Institute (ICVS), University of Minho-Campus Gualtar, 4710-057 Braga, Portugal.
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Data Brief. 2020 Nov 28;33:106598. doi: 10.1016/j.dib.2020.106598. eCollection 2020 Dec.
Here, we present the data on the biological effects of spp. and spp. plant extracts in () models of neurodegenerative diseases, which is related to the work presented in the article "Neurotherapeutic effect of spp. leaf extracts in models of tauopathy and polyglutamine disease: role of the glutathione redox cycle" [1]. This dataset was generated to define non-toxic concentrations of these plant extracts and to assess their impact on the motor phenotype and oxidative stress resistance of transgenic models of two genetically defined neurodegenerative diseases: Machado-Joseph disease and Frontotemporal dementia with Parkinsonism associated to the chromosome 17. The impact of the plant extracts on toxicity was assessed using the food-clearance assay, absorbance being measured daily for seven days at 595 nm to quantify () strain OP50 bacteria consumption. Worm length and motor behaviour, including spontaneous and stimulated movement, were analysed using videos acquired with an Olympus SZX7 stereomicroscope with an integrated camera (Olympus SC30) and processed using the Image J® software and the Wrmtrck plugin. The resistance to oxidative stress induced by 240 µM juglone was assessed by determining the percentage of live animals after 1 hour of exposure.
在此，我们展示了关于 spp. 和 spp. 植物提取物在神经退行性疾病的（）模型中的生物学效应的数据，这与文章“ spp. 叶提取物在tau蛋白病和多聚谷氨酰胺疾病模型中的神经治疗作用：谷胱甘肽氧化还原循环的作用”[1]中所呈现的工作相关。生成该数据集是为了确定这些植物提取物的无毒浓度，并评估它们对两种基因定义的神经退行性疾病的转基因 模型的运动表型和氧化应激抗性的影响：马查多 - 约瑟夫病和与17号染色体相关的伴有帕金森综合征的额颞叶痴呆。使用食物清除试验评估植物提取物对毒性的影响，连续七天每天在595nm处测量吸光度，以量化（）菌株OP50细菌的消耗。使用配备集成相机（奥林巴斯SC30）的奥林巴斯SZX7体视显微镜采集视频，并使用Image J®软件和Wrmtrck插件进行处理，分析蠕虫长度和运动行为，包括自发运动和刺激运动。通过确定暴露1小时后存活动物的百分比来评估对240µM胡桃醌诱导的氧化应激的抗性。
