用于快速检测新城疫病毒的环介导等温扩增技术
Loop-mediated isothermal amplification for rapid detection of Newcastle disease virus.
作者信息
Pham Hang Minh, Nakajima Chie, Ohashi Kazuhiko, Onuma Misao
机构信息
Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
出版信息
J Clin Microbiol. 2005 Apr;43(4):1646-50. doi: 10.1128/JCM.43.4.1646-1650.2005.
Abstract
We have evaluated a diagnostic system based on the loop-mediated isothermal amplification (LAMP) assay for the rapid, simple, and sensitive detection of Newcastle disease virus (NDV) directly from culture isolates as well as clinical samples. By using one set of specific primers targeting the fusion protein gene, the LAMP assay rapidly amplified the target gene within 2 h, requiring only a regular laboratory water bath or heat block for reaction. The results obtained from testing the genomes of 38 NDV strains, other different viruses, and clinical samples of experimentally infected chickens showed that LAMP was as sensitive and specific as nested PCR. All LAMP-positive samples were positive by nested PCR. The LAMP assay is faster than nested PCR, cost-effective, and easy to perform. Our results clearly demonstrate that the LAMP-based assay is a useful tool for the rapid and sensitive diagnosis of NDV infection.
摘要
我们评估了一种基于环介导等温扩增（LAMP）检测法的诊断系统，用于直接从培养分离株以及临床样本中快速、简便且灵敏地检测新城疫病毒（NDV）。通过使用一组靶向融合蛋白基因的特异性引物，LAMP检测法在2小时内快速扩增了目标基因，反应仅需常规实验室水浴或热块。对38株NDV毒株的基因组、其他不同病毒以及实验感染鸡的临床样本进行检测的结果表明，LAMP与巢式PCR一样灵敏且特异。所有LAMP阳性样本经巢式PCR检测均为阳性。LAMP检测法比巢式PCR更快、成本效益更高且易于操作。我们的结果清楚地表明，基于LAMP的检测法是快速灵敏诊断NDV感染的有用工具。
相似文献
1
Loop-mediated isothermal amplification for rapid detection of Newcastle disease virus.用于快速检测新城疫病毒的环介导等温扩增技术
J Clin Microbiol. 2005 Apr;43(4):1646-50. doi: 10.1128/JCM.43.4.1646-1650.2005.
2
An improved reverse transcription loop-mediated isothermal amplification assay for sensitive and specific detection of Newcastle disease virus.一种用于敏感且特异检测新城疫病毒的改进型逆转录环介导等温扩增检测方法。
Arch Virol. 2009;154(9):1433-40. doi: 10.1007/s00705-009-0464-z. Epub 2009 Aug 2.
3
Development of a reliable assay protocol for identification of diseases (RAPID)-bioactive amplification with probing (BAP) for detection of Newcastle disease virus.用于鉴定疾病的可靠检测方案（RAPID）——用于检测新城疫病毒的带探针生物活性扩增法（BAP）
Vet Microbiol. 2008 Jul 27;130(1-2):28-36. doi: 10.1016/j.vetmic.2007.12.015. Epub 2008 Jan 3.
4
Simultaneous and visual detection of infectious bronchitis virus and Newcastle disease virus by multiple LAMP and lateral flow dipstick.多重环介导等温扩增与侧向流试纸条联合可视化同步检测传染性支气管炎病毒和新城疫病毒。
Poult Sci. 2019 Nov 1;98(11):5401-5411. doi: 10.3382/ps/pez372.
5
Development of a novel real-time PCR-based strategy for simple and rapid molecular pathotyping of Newcastle disease virus.建立一种新型实时 PCR 方法，用于新城疫病毒的简单快速分子分型。
Arch Virol. 2012 May;157(5):833-44. doi: 10.1007/s00705-012-1231-0.
6
Detection and differentiation of Newcastle disease virus and influenza virus by using duplex real-time PCR.利用双重实时荧光定量PCR检测和鉴别新城疫病毒与流感病毒
Acta Biochim Pol. 2013;60(3):475-80. Epub 2013 Sep 13.
7
Assessment of Preparation of Samples Under the Field Conditions and a Portable Real-Time RT-PCR Assay for the Rapid On-Site Detection of Newcastle Disease Virus.现场条件下样本制备评估及一种用于新城疫病毒快速现场检测的便携式实时 RT-PCR 检测方法。
Transbound Emerg Dis. 2016 Apr;63(2):e245-50. doi: 10.1111/tbed.12261. Epub 2014 Sep 11.
8
Differential detection of Newcastle disease virus strains by degenerate primers based RT-PCR.基于简并引物的逆转录聚合酶链反应对新城疫病毒毒株的鉴别检测
Comp Immunol Microbiol Infect Dis. 2004 May;27(3):163-9. doi: 10.1016/j.cimid.2003.09.002.
9
Loop-mediated isothermal amplification for rapid detection of Chinese sacbrood virus.环介导等温扩增法快速检测中国囊状幼虫病毒。
J Virol Methods. 2011 Sep;176(1-2):115-9. doi: 10.1016/j.jviromet.2011.05.028. Epub 2011 May 27.
10
Sensitive, semi-nested RT-PCR amplification of fusion gene sequences for the rapid detection and differentiation of Newcastle disease virus.用于新城疫病毒快速检测和区分的融合基因序列的敏感、半巢式 RT-PCR 扩增。
Res Vet Sci. 2010 Oct;89(2):282-9. doi: 10.1016/j.rvsc.2010.02.007. Epub 2010 Mar 9.
引用本文的文献
1
Point-of-Care Diagnostic Testing for Emerging and Existing Poultry Viral Respiratory Pathogens Using Loop-Mediated Isothermal Amplification.使用环介导等温扩增技术对新出现和现存的家禽病毒性呼吸道病原体进行即时诊断检测。
Pathogens. 2025 Jul 2;14(7):657. doi: 10.3390/pathogens14070657.
2
A snapshot on molecular technologies for diagnosing FAdV infections.用于诊断禽腺病毒感染的分子技术概述。
Front Vet Sci. 2025 Apr 30;12:1558257. doi: 10.3389/fvets.2025.1558257. eCollection 2025.
3
Molecular Detection of Respiratory Tract Viruses in Chickens at the Point of Need by Loop-Mediated Isothermal Amplification (LAMP).基于环介导等温扩增（LAMP）的即时检测技术在禽类呼吸道病毒的分子检测中的应用
Viruses. 2024 Aug 3;16(8):1248. doi: 10.3390/v16081248.
4
5
Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP.使用 RT-LAMP 分子信标检测和区分低毒力和毒力 Orthoavulavirus javaense。
Sci Rep. 2024 Aug 5;14(1):18047. doi: 10.1038/s41598-024-68816-7.
6
DNA-directed formation of plasmonic core-satellite nanostructures for quantification of hepatitis C viral RNA.用于丙型肝炎病毒RNA定量的基于DNA导向的等离子体核-卫星纳米结构的形成。
Chem Sci. 2024 Apr 22;15(21):8112-8126. doi: 10.1039/d4sc00891j. eCollection 2024 May 29.
7
The Development of Novel Reverse Transcription Loop-Mediated Isothermal Amplification Assays for the Detection and Differentiation of Virulent Newcastle Disease Virus.新型逆转录环介导等温扩增检测与鉴别新城疫病毒方法的建立。
Int J Mol Sci. 2023 Sep 8;24(18):13847. doi: 10.3390/ijms241813847.
8
Monitoring of Newcastle Disease Virus Vaccine Strain Replication in Embryonated Chicken Eggs by Reverse Transcription-Polymerase Chain Reaction.用反转录-聚合酶链反应监测鸡胚中新城疫病毒疫苗株的复制。
Arch Razi Inst. 2023 Apr 30;78(2):767-773. doi: 10.22092/ARI.2022.359142.2377. eCollection 2023 Apr.
9
Review detection of Newcastle disease virus.新城疫病毒检测综述。
Front Vet Sci. 2022 Aug 2;9:936251. doi: 10.3389/fvets.2022.936251. eCollection 2022.
10
Diagnosis of visceral and cutaneous leishmaniasis using loop-mediated isothermal amplification (LAMP) protocols: a systematic review and meta-analysis.应用环介导等温扩增（LAMP）技术诊断内脏利什曼病和皮肤利什曼病：一项系统评价和荟萃分析。
Parasit Vectors. 2022 Jan 24;15(1):34. doi: 10.1186/s13071-021-05133-2.
本文引用的文献
1
Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1).新城疫病毒（禽副黏病毒 1 型）的检测与鉴别。
Avian Pathol. 2001 Apr;30(2):117-28. doi: 10.1080/03079450120044515.
2
Detection of Newcastle disease virus in organs and faeces of experimentally infected chickens using RT-PCR.应用 RT-PCR 检测实验感染鸡的器官和粪便中的新城疫病毒。
Avian Pathol. 2000 Apr;29(2):143-52. doi: 10.1080/03079450094171.
3
Newcastle disease in countries of the European Union.
Avian Pathol. 1995 Mar;24(1):3-10. doi: 10.1080/03079459508419045.
4
Newcastle disease virus: Detection and characterization by PCR of recent German isolates differing in pathogenicity.新城疫病毒：通过 PCR 对德国近期分离株进行致病性差异检测和特征分析。
Avian Pathol. 1998;27(3):237-43. doi: 10.1080/03079459808419330.
5
Differentiation of virulent and non-virulent strains of Newcastle disease virus within 24 hours by polymerase chain reaction.24 小时内通过聚合酶链反应区分新城疫病毒的毒力和非毒力株。
Avian Pathol. 1997;26(4):837-49. doi: 10.1080/03079459708419257.
6
Antigenic diversity and similarities detected in avian paramyxovirus type 1 (Newcastle disease virus) isolates using monoclonal antibodies.使用单克隆抗体检测到禽副黏病毒 1 型（新城疫病毒）分离株的抗原多样性和相似性。
Avian Pathol. 1997;26(2):399-418. doi: 10.1080/03079459708419222.
7
Development of a real-time reverse-transcription PCR for detection of newcastle disease virus RNA in clinical samples.一种用于检测临床样本中新城疫病毒RNA的实时逆转录PCR技术的开发。
J Clin Microbiol. 2004 Jan;42(1):329-38. doi: 10.1128/JCM.42.1.329-338.2004.
8
Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples.用于直接检测痰液样本中结核分枝杆菌复合群、鸟分枝杆菌和胞内分枝杆菌的环介导等温扩增技术。
J Clin Microbiol. 2003 Jun;41(6):2616-22. doi: 10.1128/JCM.41.6.2616-2622.2003.
9
Development of a RT-nested PCR test detecting pigeon Paramyxovirus-1 directly from organs of infected animals.一种直接从感染动物器官中检测鸽副粘病毒-1的逆转录巢式聚合酶链反应检测方法的开发。
J Virol Methods. 2002 Dec;106(2):197-207. doi: 10.1016/s0166-0934(02)00148-9.
10
Phylogenetic analysis of Newcastle disease virus genotypes isolated in Japan.对在日本分离出的新城疫病毒基因型的系统发育分析。
J Clin Microbiol. 2002 Oct;40(10):3826-30. doi: 10.1128/JCM.40.10.3826-3830.2002.
Zotero 插件