Proteomics Research Unit, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Section of Cell Biology and Biophysics, Department of Biology, National and Kapodistrian University of Athens, Athens, Greece.
Cancer Genomics Proteomics. 2020 Nov-Dec;17(6):757-767. doi: 10.21873/cgp.20230.
BACKGROUND/AIM: Proteomics technologies provide fundamental insights into the high organizational complexity and diversity of the central nervous system. In the present study, high-resolution mass spectrometry (MS) was applied in order to identify whole-proteome content of anatomically distinct and functionally specific mouse brain regions.
Brains from eight 8-week-old C57BL/6N normal male mice were separated into seven anatomically district regions. The protein content of each region was analyzed by high-throughput nano-liquid chromatography-MS/MS Orbitrap elite technology.
A total of 16,574 proteins were identified: 2,795 in cerebral cortex, 2,311 in olfactory bulb, 2,246 in hippocampus, 2,247 in hypothalamus, 2,250 in mid brain, 2,334 in cerebellum and 2,391 in medulla. Of these proteins, 534 were uniquely expressed in cerebral cortex, 323 in olfactory bulb, 230 in hippocampus, 272 in hypothalamus, 1,326 in mid brain, 320 in cerebellum and 268 in medulla.
These data represent the most comprehensive proteomic map of the normal mouse brain and they might further be used in studies related to brain diseases, including cancer and neurodegenerative diseases.
背景/目的：蛋白质组学技术为深入了解中枢神经系统的高度组织复杂性和多样性提供了基础。本研究应用高分辨率质谱（MS）技术，以鉴定解剖学上不同和功能特异的小鼠脑区的全蛋白质组内容。
将 8 只 8 周龄 C57BL/6N 正常雄性小鼠的大脑分离成 7 个解剖区域。采用高通量纳流色谱-MS/MS Orbitrap elite 技术分析每个区域的蛋白质含量。
共鉴定到 16574 种蛋白质：大脑皮层 2795 种，嗅球 2311 种，海马体 2246 种，下丘脑 2247 种，中脑 2250 种，小脑 2334 种，延髓 2391 种。其中，534 种蛋白质在大脑皮层中特异性表达，323 种在嗅球中，230 种在海马体中，272 种在下丘脑，1326 种在中脑中，320 种在小脑，268 种在延髓。
这些数据代表了正常小鼠大脑最全面的蛋白质组图谱，它们可能进一步用于与癌症和神经退行性疾病等脑疾病相关的研究。
