高通量蛋白质组学分析男性乳腺癌组织。
High-throughput Proteomic Profiling of Male Breast Cancer Tissue.
机构信息
Department of Basic Medical Sciences, Laboratory of Biology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece;
Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
出版信息
Cancer Genomics Proteomics. 2022 Mar-Apr;19(2):229-240. doi: 10.21873/cgp.20316.
BACKGROUND/AIM: Until now, little emphasis has been placed on the protein expression profile of male breast cancer (MBC) tumors, due to the rarity of the disease. The present study aimed to identify a proteomic pattern that is characteristic for malignant male breast tissue epithelium.
MATERIALS AND METHODS
The protein content of four male breast tumors and corresponding adjacent healthy (control) tissues was analyzed by high-throughput nano-liquid chromatography-MS/MS technology.
RESULTS
A total of 2,352 proteins were identified, that correspond to 1,249 single gene products, with diverse biological roles. Of those, a panel of 119 differentially expressed tissue proteins was identified in MBC samples compared to controls; 90 were found to be over-expressed in MBC tissues, while 29 were down-regulated. Concurrently, 844 proteins were detected only in MBC tumors and 197 were expressed exclusively in control mammary samples.
CONCLUSION
Differential proteomic expression was found in MBC tissue, leading to improved understanding of MBC pathology and highlighting the need for personalized management of male patients.
背景/目的：由于男性乳腺癌（MBC）疾病的罕见性，目前对于 MBC 肿瘤的蛋白表达谱还没有得到太多关注。本研究旨在确定一种可用于恶性男性乳腺组织上皮的蛋白质组学模式。
材料与方法
采用高通量纳流色谱-MS/MS 技术分析了 4 例男性乳腺癌肿瘤及相应的相邻正常（对照）组织中的蛋白质含量。
结果
共鉴定出 2352 种蛋白质，对应 1249 种单一基因产物，具有多种生物学功能。在 MBC 样本与对照相比，鉴定出一组 119 种差异表达的组织蛋白；90 种在 MBC 组织中过表达，而 29 种则下调。同时，在 MBC 肿瘤中检测到 844 种蛋白，而在对照乳腺样本中仅表达 197 种蛋白。
结论
MBC 组织中存在差异蛋白质组表达，从而提高了对 MBC 病理学的认识，并强调了对男性患者进行个体化管理的必要性。
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