Ito Y, Kitazume E, Bhatnagar M, Trimble F D
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
J Chromatogr. 1991 Jan 18;538(1):59-66. doi: 10.1016/s0021-9673(01)91621-8.
The fourth prototype holds a pair of column holders in the lateral position at 15 cm from the center of the rotary shaft horizontally mounted on the rotary frame at 7.6 cm from the central axis of the apparatus. Using short coils of 2.6 mm I.D. PTFE (polytetrafluoroethylene) tubing with 7.6 cm and 24 cm helical diameters, retention of the stationary phase was measured in ten pairs of two-phase solvent systems under various experimental conditions. Satisfactory retention was obtained by choosing proper combinations of three factors, i.e., the direction of planetary motion, head-tail elution mode, and inward-outward elution mode. The polar butanol solvent systems showed excellent retention from 65 to 80% in the 7.6 cm helical diameter left-handed coil.
第四个原型在水平安装在旋转框架上距设备中心轴7.6厘米处的旋转轴中心水平15厘米的横向位置持有一对柱架。使用内径为2.6毫米的聚四氟乙烯（PTFE）短管，螺旋直径分别为7.6厘米和24厘米，在各种实验条件下，在十对两相溶剂系统中测量固定相的保留情况。通过选择行星运动方向、头尾洗脱模式和内外洗脱模式这三个因素的适当组合，获得了令人满意的保留率。极性丁醇溶剂系统在7.6厘米螺旋直径的左旋线圈中显示出65%至80%的优异保留率。
