Wang Juan, Zhou Haixin
College of Physical Education and Health, Zhaoqing University, Zhaoqing 526061, China.
Physical Education College, Guangxi University of Science and Technology, Liuzhou 545006, China.
Saudi J Biol Sci. 2020 Nov;27(11):3145-3149. doi: 10.1016/j.sjbs.2020.07.014. Epub 2020 Jul 14.
The paper intends to study the protective effects of sulforaphane (SF) on acute alcoholic hepatic injury in mice by intragastric administration of SF, aerobic exercise and the approach of SF integrated with aerobic exercise.
60 NIH mice were randomly divided into 6 groups of equal number according to their body weight and were intragastrically administrated with 50% ethanol. The serum and liver indexes of each group of mice were detected, and the liver was stained with oil red O for pathological examination.
Compared with the model group, the serum TG and the ratio of liver to body weight of the model mice that suffered from acute alcoholic hepatic injury could be significantly decreased in the group that practiced aerobic exercise, the group administered with SF, and the group treated with the approach of SF integrated with aerobic exercise ( < 0.05). The contents of TG and MDA in liver could be significantly decreased ( < 0.05) and SOD activity could be significantly increased ( < 0.05) both in the group administered with SF and the group treated with the approach of SF integrated with aerobic exercise. Serum VLDL ( < 0.05) could also be significantly reduced in the group treated with the approach of SF integrated with aerobic exercise.
Both SF and aerobic exercise could alleviate alcohol-induced acute alcoholic hepatic injury in mice possibly thanks to the working mechanism related to antioxidant stress that reduced the harm posed by alcohol on hepatic cells. In addition, the protective effect of SF on acute alcoholic hepatic injury in mice was stronger than that of aerobic exercise, while the approach of SF integrated with aerobic exercise had the strongest protective effect on acute alcoholic hepatic injury in mice.
通过对小鼠灌胃给予萝卜硫素（SF）、有氧运动以及SF与有氧运动相结合的方式，研究萝卜硫素对小鼠急性酒精性肝损伤的保护作用。
将60只NIH小鼠按体重随机等分为6组，给予50%乙醇灌胃。检测每组小鼠的血清和肝脏指标，并用油红O对肝脏进行染色以进行病理检查。
与模型组相比，有氧运动组、SF给药组以及SF与有氧运动相结合处理组的急性酒精性肝损伤模型小鼠的血清甘油三酯（TG）和肝体比均显著降低（<0.05）。SF给药组和SF与有氧运动相结合处理组肝脏中TG和丙二醛（MDA）含量均显著降低（<0.05），超氧化物歧化酶（SOD）活性显著升高（<0.05）。SF与有氧运动相结合处理组血清极低密度脂蛋白（VLDL）也显著降低（<0.05）。
SF和有氧运动均可减轻酒精诱导的小鼠急性酒精性肝损伤，这可能得益于其与抗氧化应激相关的作用机制，减少了酒精对肝细胞的损害。此外，SF对小鼠急性酒精性肝损伤的保护作用强于有氧运动，而SF与有氧运动相结合的方式对小鼠急性酒精性肝损伤的保护作用最强。
