云母可通过减少血管紧张素 II 和白介素-17A 并增加血管紧张素转换酶 2、血管紧张素 1-7 和白介素-10 来缓解小鼠 TNBS 诱导的结肠炎。
Mica Can Alleviate TNBS-Induced Colitis in Mice by Reducing Angiotensin II and IL-17A and Increasing Angiotensin-Converting Enzyme 2, Angiotensin 1-7, and IL-10.
机构信息
Department of Internal Medicine, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006 Zhejiang Province, China.
Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou City, Zhejiang Province 310006, China.
出版信息
Mediators Inflamm. 2020 Oct 10;2020:3070345. doi: 10.1155/2020/3070345. eCollection 2020.
AIM
To explore the treatment effect of mica on 2,4,6-trinitrobenzenesulfonic acid solution- (TNBS-) induced colitis in mice.
MATERIALS AND METHODS
Thirty male BALB/C mice were randomly divided into the control group, the TNBS group, and the mica group. Control mice were treated with saline solution. Experimental colitis was induced by TNBS (250 mg/kg/d) in the TNBS group and the mica group. After modeling, the mica group was treated with mica (180 mg/kg/d) for 3 days, while the TNBS group continued the treatment with TNBS. All solutions were injected intrarectally. During treatment, body weight and mice activity were monitored daily. After treatment, the colon tissues of mice were collected; angiotensin II (Ang II), angiotensin-converting enzyme 2 (ACE2), angiotensin 1-7 (Ang (1-7)), IL-17A, and IL-10 expression was analyzed by ELISA and immunohistochemistry.
RESULTS
Food intake, activity, and body weight gradually decreased in the TNBS group compared to the control group and the mica group (all < 0.05). Also, black stool adhesion in the anus and thin and bloody stool were observed in the TNBS group, but not in the other two groups. Moreover, the expression of Ang II, ACE2, Ang (1-7), IL-17A, and IL-10 in the TNBS group increased compared to that in the control group. Compared to the TNBS group, ACE2, Ang (1-7), and IL-10 in the mica group increased, while Ang II and IL-17A decreased (all < 0.05).
CONCLUSION
Mica can alleviate TNBS-induced colitis in mice by regulating the inflammation process; it reduces Ang II and IL-17A and increases ACE2, IL-10, and Ang (1-7).
目的
探讨云母对 2,4,6-三硝基苯磺酸溶液-（TNBS-）诱导的小鼠结肠炎的治疗作用。
材料与方法
30 只雄性 BALB/C 小鼠随机分为对照组、TNBS 组和云母组。对照组给予生理盐水处理。TNBS 组和云母组采用 TNBS（250mg/kg/d）诱导实验性结肠炎。造模后，云母组给予云母（180mg/kg/d）治疗 3 天，TNBS 组继续给予 TNBS 治疗。所有溶液均经直肠内注射。治疗期间，每日监测体质量和小鼠活动度。治疗结束后，收集小鼠结肠组织；采用 ELISA 和免疫组化法检测血管紧张素 II（Ang II）、血管紧张素转换酶 2（ACE2）、血管紧张素 1-7（Ang（1-7））、白细胞介素 17A（IL-17A）和白细胞介素 10（IL-10）的表达。
结果
与对照组和云母组相比，TNBS 组的摄食量、活动度和体质量逐渐下降（均<0.05）。此外，TNBS 组可见肛门处有黑便黏附，大便稀薄且带血，而其他两组未见此现象。与对照组相比，TNBS 组的 Ang II、ACE2、Ang（1-7）、IL-17A 和 IL-10 表达增加。与 TNBS 组相比，云母组的 ACE2、Ang（1-7）和 IL-10 增加，而 Ang II 和 IL-17A 减少（均<0.05）。
结论
云母通过调节炎症过程减轻 TNBS 诱导的小鼠结肠炎，其减少 Ang II 和 IL-17A，增加 ACE2、IL-10 和 Ang（1-7）。
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