Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
Department of Orthopedics, Peking University Shougang Hospital, Beijing, China.
Front Endocrinol (Lausanne). 2020 Sep 25;11:571549. doi: 10.3389/fendo.2020.571549. eCollection 2020.
The study aimed to explore the associations of rs4988300 and rs634008 in the low-density lipoprotein receptor-related protein 5 () gene with bone mineral density (BMD), bone turnover markers (BTM), and fractures in elderly patients with osteoporosis (OP). Our study included 328 unrelated OP patients with or without fractures. Genomic DNA was extracted for genotyping. BTM levels were assessed by electrochemiluminescence (ECL). Dual-energy X-ray absorptiometry (DXA) was employed to measure BMD in the lumbar spine (LS) and proximal femur. Basic features between the OP and fracture groups were analyzed using the -test. The Chi-square test was performed to analyze the differences in allele and genotype frequencies. The associations of single-nucleotide polymorphisms (SNPs) with BMD and BTM in the subgroups were investigated by the analysis of covariance (ANCOVA) adjusted for confounding factors. In both females and males, individuals with fractures exhibited higher BTM levels and lower BMD values than those with OP ( < 0.05). The allele and genotype frequencies of rs4988300 in the subgroups were significantly different ( < 0.05). In both females and males suffering from OP, participants with rs4988300 GG or rs634008 TT presented lower procollagen I N-terminal propeptide (PINP) levels ( < 0.05). Women with OP carrying rs4988300 GG exhibited lower BMD values at FN and TH ( < 0.05). In both females and males with fractures, individuals carrying rs4988300 GG genotype or rs634008 TT genotype exhibited lower PINP levels and BMD values at FN and TH than those with other genotypes ( < 0.05). Rs4988300 and rs634008 polymorphisms in the gene are associated with bone phenotypes in the elderly with OP or fractures.
本研究旨在探讨载脂蛋白脂蛋白受体相关蛋白 5()基因中的 rs4988300 和 rs634008 与老年骨质疏松症（OP）患者的骨密度（BMD）、骨转换标志物（BTM）和骨折之间的关联。我们的研究纳入了 328 例无骨折或有骨折的无关 OP 患者。提取基因组 DNA 进行基因分型。采用电化学发光（ECL）法检测 BTM 水平。采用双能 X 线吸收法（DXA）测量腰椎（LS）和股骨近端的 BMD。采用 t 检验分析 OP 组和骨折组之间的基本特征。采用卡方检验分析等位基因和基因型频率的差异。采用协方差分析（ANCOVA）调整混杂因素后，分析单核苷酸多态性（SNP）与亚组中 BMD 和 BTM 的关系。在女性和男性中，骨折组的 BTM 水平更高，BMD 值更低（<0.05）。亚组中 rs4988300 的等位基因和基因型频率差异有统计学意义（<0.05）。在患有 OP 的女性和男性中，rs4988300 GG 或 rs634008 TT 携带者的前胶原 I N 端前肽（PINP）水平较低（<0.05）。携带 rs4988300 GG 的女性 OP 患者 FN 和 TH 的 BMD 值较低（<0.05）。在女性和男性骨折患者中，携带 rs4988300 GG 基因型或 rs634008 TT 基因型的个体的 PINP 水平和 FN 和 TH 的 BMD 值均低于其他基因型的个体（<0.05）。基因中的 rs4988300 和 rs634008 多态性与老年 OP 或骨折患者的骨骼表型有关。
