The Joint Center for Infection and Immunity, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
Institut Pasteur of Shanghai, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
Front Immunol. 2020 Sep 25;11:577546. doi: 10.3389/fimmu.2020.577546. eCollection 2020.
Japanese encephalitis virus (JEV) exposure or vaccination could elicit cross-reactive CD8 T cell immunity against heterologous flaviviruses in humans. In addition, cross-reactive CD8 T cells induced by dengue virus (DENV) have been shown to play a protective role against Zika virus (ZIKV). However, how JEV exposure or vaccination affects ZIKV infection in humans remains unclear. In this report, epitope prediction algorithms were used to predict the cross-reactive CD8 T cell epitope restricted to human HLA between JEV and ZIKV. We found that these predicted CD8 T cell epitopes are immunogenic and cross-reactive in humanized HLA transgenic mice. Moreover, JEV vaccine immunization provided cross-protection against ZIKV infection. Furthermore, CD8 T cells were involved in the protection against ZKIV infection . Our results have an important clinical implication that vaccination with JEV SA14-14-2 may provide protection against ZIKV infection in humans.
日本脑炎病毒（JEV）暴露或接种可在人类中引起针对异源黄病毒的交叉反应性 CD8 T 细胞免疫。此外，登革热病毒（DENV）诱导的交叉反应性 CD8 T 细胞已被证明对寨卡病毒（ZIKV）具有保护作用。然而，JEV 暴露或接种如何影响人类的 ZIKV 感染尚不清楚。在本报告中，使用表位预测算法来预测 JEV 和 ZIKV 之间限制于人 HLA 的交叉反应性 CD8 T 细胞表位。我们发现这些预测的 CD8 T 细胞表位在人源化 HLA 转基因小鼠中具有免疫原性和交叉反应性。此外，JEV 疫苗免疫接种可提供针对 ZIKV 感染的交叉保护。此外，CD8 T 细胞参与了对 ZKIV 感染的保护。我们的结果具有重要的临床意义，即用 JEV SA14-14-2 进行疫苗接种可能为人类提供对 ZIKV 感染的保护。
