Fan Kai, Mao Zhijun, Zheng Jiaxin, Chen Yunrui, Li Zhaowei, Lin Weiwei, Zhang Yongqiang, Huang Jinwen, Lin Wenxiong
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China.
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Plant Sci. 2020 Sep 30;11:545042. doi: 10.3389/fpls.2020.545042. eCollection 2020.
The comprehensive analysis of gene family evolution will elucidate the origin and evolution of gene families. The K+ uptake (KUP) gene family plays important roles in K+ uptake and transport, plant growth and development, and abiotic stress responses. However, the current understanding of the KUP family in cotton is limited. In this study, 51 and 53 KUPs were identified in and , respectively. These KUPs were divided into five KUP subfamilies, with subfamily 2 containing three groups. Different subfamilies had different member numbers, conserved motifs, gene structures, regulatory elements, and gene expansion and loss rates. A paleohexaploidization event caused the expansion of GhKUP and GbKUP in cotton, and duplication events in and have happened in a common ancestor of . Meanwhile, the KUP members of the two allopolyploid subgenomes of and exhibited unequal gene proportions, gene structural diversity, uneven chromosomal distributions, asymmetric expansion rates, and biased gene loss rates. In addition, the KUP families of and displayed evolutionary conservation and divergence. Taken together, these results illustrated the molecular evolution and expansion of the KUP family in allopolyploid cotton species.
基因家族进化的综合分析将阐明基因家族的起源和进化。钾离子吸收（KUP）基因家族在钾离子吸收与转运、植物生长发育以及非生物胁迫响应中发挥着重要作用。然而，目前对棉花中KUP家族的了解有限。在本研究中，分别在[具体物种1]和[具体物种2]中鉴定出51个和53个KUP基因。这些KUP基因被分为五个KUP亚家族，其中亚家族2包含三个组。不同亚家族的成员数量、保守基序、基因结构、调控元件以及基因扩增和丢失率各不相同。一次古六倍体化事件导致了棉花中GhKUP和GbKUP的扩增，并且[具体物种1]和[具体物种2]中的重复事件发生在[共同祖先物种]的一个共同祖先中。同时，[具体物种1]和[具体物种2]的两个异源多倍体亚基因组的KUP成员表现出基因比例不均、基因结构多样性、染色体分布不均、扩增速率不对称以及基因丢失率有偏差。此外，[具体物种1]和[具体物种2]的KUP家族表现出进化上的保守性和分歧性。综上所述，这些结果阐明了异源多倍体棉花物种中KUP家族的分子进化和扩增情况。
