Wang Meng, Gong Jiazhen, Bhullar Navreet K
School of Life Sciences, University of Science and Technology of China, Hefei, China.
Institute of Molecular Plant Biology, Department of Biology, ETH Zurich (Swiss Federal Institute of Technology), Zurich, Switzerland.
Comput Struct Biotechnol J. 2020 Sep 20;18:2709-2722. doi: 10.1016/j.csbj.2020.09.009. eCollection 2020.
A series of complex transport, storage and regulation mechanisms control iron metabolism and thereby maintain iron homeostasis in plants. Despite several studies on iron deficiency responses in different plant species, these mechanisms remain unclear in the allohexaploid wheat, which is the most widely cultivated commercial crop. We used RNA sequencing to reveal transcriptomic changes in the wheat flag leaves and roots, when subjected to iron limited conditions. We identified 5969 and 2591 differentially expressed genes (DEGs) in the flag leaves and roots, respectively. Genes involved in the synthesis of iron ligands i.e., nicotianamine (NA) and deoxymugineic acid (DMA) were significantly up-regulated during iron deficiency. In total, 337 and 635 genes encoding transporters exhibited altered expression in roots and flag leaves, respectively. Several genes related to (), () transporter superfamily, () family and () family were regulated, indicating their important roles in combating iron deficiency stress. Among the regulatory factors, the genes encoding for transcription factors of () family were highly up-regulated in both roots and the flag leaves. The jasmonate biosynthesis pathway was significantly altered but with notable expression differences between roots and flag leaves. Homoeologs expression and induction bias analysis revealed subgenome specific differential expression. Our findings provide an integrated overview on regulated molecular processes in response to iron deficiency stress in wheat. This information could potentially serve as a guideline for breeding iron deficiency stress tolerant crops as well as for designing appropriate wheat iron biofortification strategies.
一系列复杂的运输、储存和调节机制控制着植物中的铁代谢，从而维持植物体内的铁稳态。尽管对不同植物物种的缺铁反应进行了多项研究，但在最广泛种植的商业作物异源六倍体小麦中，这些机制仍不清楚。我们使用RNA测序来揭示小麦旗叶和根在铁限制条件下的转录组变化。我们分别在旗叶和根中鉴定出5969个和2591个差异表达基因（DEG）。在缺铁期间，参与铁配体即烟酰胺（NA）和脱氧 mugineic 酸（DMA）合成的基因显著上调。总共，分别有337个和635个编码转运蛋白的基因在根和旗叶中表达发生改变。几个与（）、（）转运蛋白超家族、（）家族和（）家族相关的基因受到调控，表明它们在对抗缺铁胁迫中发挥重要作用。在调控因子中，编码（）家族转录因子的基因在根和旗叶中均高度上调。茉莉酸生物合成途径发生了显著改变，但根和旗叶之间存在明显的表达差异。同源基因表达和诱导偏向分析揭示了亚基因组特异性差异表达。我们的研究结果提供了小麦对缺铁胁迫响应中调控分子过程的综合概述。这些信息可能为培育耐缺铁胁迫作物以及设计合适的小麦铁生物强化策略提供指导。
