College of Life Science and Technology, Harbin Normal University, Harbin 150025, China.
Institute of Crops Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.
Int J Mol Sci. 2023 Jul 10;24(14):11278. doi: 10.3390/ijms241411278.
Iron(Fe) is a trace metal element necessary for plant growth, but excess iron is harmful to plants. Natural resistance-associated macrophage proteins (NRAMPs) are important for divalent metal transport in plants. In this study, we isolated the (MN_547960) gene from alfalfa, the perennial legume forage. The expression of is specifically induced by iron excess. Overexpression of conferred transgenic tobacco tolerance to iron excess, while it conferred yeast sensitivity to excess iron. Together with the gene, (MN_547959) expression is induced by excess iron. Y1H indicated that the MsMYB protein could bind to the "CTGTTG" cis element of the promoter. The results indicated that has a function in iron transport and its expression might be regulated by . The excess iron tolerance ability enhancement of may be involved in iron transport, sequestration, or redistribution.
铁（Fe）是植物生长所必需的痕量金属元素，但过量的铁对植物有害。天然抗性相关巨噬细胞蛋白（NRAMPs）是植物中二价金属运输的重要物质。在这项研究中，我们从紫花苜蓿中分离出 （MN_547960）基因，紫花苜蓿是一种多年生豆科牧草。 的表达受铁过量的特异性诱导。 的过表达赋予转基因烟草对铁过量的耐受性，而赋予酵母对过量铁的敏感性。与 基因一起， （MN_547959）的表达受铁过量诱导。Y1H 表明 MsMYB 蛋白可以与 启动子的“CTGTTG”顺式元件结合。结果表明， 在铁运输中具有功能，其表达可能受到 的调节。 增强对过量铁的耐受性能力可能涉及铁的运输、螯合或再分配。
