Garagounis Constantine, Georgopoulou Maria-Eleni, Beritza Konstantina, Papadopoulou Kalliope K
University of Thessaly, Department of Biochemistry and Biotechnology, Biopolis, Larisa 41500, Greece.
MethodsX. 2020 Oct 9;7:101098. doi: 10.1016/j.mex.2020.101098. eCollection 2020.
•This work describes a protocol for hairy root transformation of the medicinal crop legume fenugreek (). Hairy root plant transformation mediated by is an established method for the rapid genetic transformation of various dicotyledonous plants. We have adapted a hairy root transformation protocol from the model legume for use in this metabolically rich non-model crop legume. Considering the great variety and abundance of phytochemicals in fenugreek and its established use in traditional medicine, we aim for this method to become a resource for metabolic pathway identification and for production of valuable specialised metabolites via metabolic engineering approaches.•Development rapid transformation (2.5-3 weeks) of fenugreek roots via •Marker gene cassette with suitable promoter for visual detection of transformed fenugreek roots.
•本研究描述了一种用于药用作物豆科胡芦巴（）毛状根转化的方案。由介导的毛状根植物转化是一种用于多种双子叶植物快速遗传转化的成熟方法。我们从模式豆科植物改编了一种毛状根转化方案，用于这种富含代谢物的非模式豆科作物。鉴于胡芦巴中植物化学物质的种类繁多且含量丰富，以及其在传统医学中的既定用途，我们旨在使该方法成为通过代谢工程方法鉴定代谢途径和生产有价值的特殊代谢物的资源。•通过实现胡芦巴根的快速转化（2.5 - 3周）
•带有合适启动子的标记基因盒，用于可视化检测转化的胡芦巴根。
