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PGLa 和 Magainin 2 可逆的非共价纤维聚集能保持它们的抗菌活性和协同作用。

The Reversible Non-covalent Aggregation Into Fibers of PGLa and Magainin 2 Preserves Their Antimicrobial Activity and Synergism.

机构信息

University of Strasbourg/CNRS, UMR7177, Institut de Chimie de Strasbourg, Strasbourg, France.

Institut Universitaire de France (IUF), Paris, France.

出版信息

Front Cell Infect Microbiol. 2020 Sep 30;10:526459. doi: 10.3389/fcimb.2020.526459. eCollection 2020.

DOI:10.3389/fcimb.2020.526459
PMID:33102247
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7554302/
Abstract

Magainin 2 and PGLa are antimicrobial peptides found together in frog skin secretions. When added as a mixture they show an order of magnitude increase in antibacterial activity and in model membrane permeation assays. Here we demonstrate that both peptides can form fibers with beta-sheet/turn signature in ATR-FTIR- and CD-spectroscopic analyses, but with different morphologies in EM images. Whereas, fiber formation results in acute reduction of the antimicrobial activity of the individual peptides, the synergistic enhancement of activity remains for the equimolar mixture of PGLa and magainin 2 also after fibril formation. The biological significance and potential applications of such supramolecular aggregates are discussed.

摘要

Magainin 2 和 PGLa 是在青蛙皮肤分泌物中发现的两种抗菌肽。当它们混合在一起时，抗菌活性会提高一个数量级，并且在模型膜渗透测定中也是如此。在这里，我们证明，在 ATR-FTIR 和 CD 光谱分析中，这两种肽都可以形成具有β-折叠/转角特征的纤维，但在 EM 图像中呈现出不同的形态。然而，纤维的形成导致单个肽的抗菌活性急剧降低，但在纤维形成后，PGLa 和 magainin 2 的等摩尔混合物的协同增强活性仍然存在。讨论了这种超分子聚集体的生物学意义和潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ed2/7554302/62254b2fc013/fcimb-10-526459-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ed2/7554302/4f954da79046/fcimb-10-526459-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ed2/7554302/171f1bdafcd2/fcimb-10-526459-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ed2/7554302/62254b2fc013/fcimb-10-526459-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ed2/7554302/4f954da79046/fcimb-10-526459-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ed2/7554302/171f1bdafcd2/fcimb-10-526459-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ed2/7554302/62254b2fc013/fcimb-10-526459-g0003.jpg

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