Raveschot Cyril, Deracinois Barbara, Bertrand Emmeline, Flahaut Christophe, Frémont Marc, Drider Djamel, Dhulster Pascal, Cudennec Benoit, Coutte François
UMR Transfrontalière BioEcoAgro N°1158, Université de Lille, INRAE, Université de Liège, UPJV, YNCREA, Université d'Artois, Université du Littoral Côte d'Opale, ICV - Institut Charles Viollette, Lille, France.
VF Bioscience, Loos-lez-Lille, France.
Front Bioeng Biotechnol. 2020 Sep 25;8:585815. doi: 10.3389/fbioe.2020.585815. eCollection 2020.
Production of bioactive peptides (BAPs) by species is a cost-effective approach compared to the use of purified enzymes. In this study, proteolytic strains were used for milk fermentation to produce BAPs capable of inhibiting angiotensin converting enzyme (ACE). Fermented milks were produced in bioreactors using batch mode, and the resulting products showed significant ACE-inhibitory activities. However, the benefits of fermentation in terms of peptide composition and ACE-inhibitory activity were noticeably reduced when the samples (fermented milks and non-fermented controls) were subject to simulated gastrointestinal digestion (GID). Introducing an ultrafiltration step after fermentation allowed to prevent this effect of GID and restored the effect of fermentation. Furthermore, an integrated continuous process for peptide production was developed which led to a 3 fold increased peptide productivity compared to batch production. Using a membrane bioreactor allowed to generate and purify in a single step, an active ingredient for ACE inhibition.
与使用纯化酶相比，利用菌种生产生物活性肽（BAPs）是一种经济高效的方法。在本研究中，蛋白水解菌株被用于牛奶发酵以生产能够抑制血管紧张素转换酶（ACE）的BAPs。在生物反应器中采用分批模式生产发酵乳，所得产品显示出显著的ACE抑制活性。然而，当样品（发酵乳和未发酵对照）进行模拟胃肠道消化（GID）时，发酵在肽组成和ACE抑制活性方面的益处明显降低。在发酵后引入超滤步骤可防止GID的这种影响并恢复发酵效果。此外，还开发了一种肽生产的集成连续工艺，与分批生产相比，该工艺使肽生产率提高了3倍。使用膜生物反应器可在一步中生成并纯化用于ACE抑制的活性成分。
