Smith G L, Levin J Z, Palese P, Moss B
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Virology. 1987 Oct;160(2):336-45. doi: 10.1016/0042-6822(87)90004-3.
A complete set of recombinant vaccinia viruses that express each of the influenza virus polypeptides has been constructed. PB1, PB2, PA, HA, NP, M1, and NS1 genes were derived from influenza virus A/PR/8/34, NA from influenza virus A/Cam/46, and M2 and NS2 genes from influenza virus A/Udorn/72. Cells infected with these recombinant viruses synthesize influenza polypeptides that are precipitable with specific antisera and that have electrophoretic mobilities similar to the corresponding influenza virus polypeptides. Indirect immunofluorescence studies have shown that HA, NA, and MS2 proteins migrate to the cell surface; PB2, PB1, PA, NP, and NS1 proteins migrate to the cell nucleus; and M1 and NS2 are distributed throughout the cell, although NS2 accumulates preferentially in nuclei. These transport processes occurred independently of other influenza polypeptides and are therefore attributable to the intrinsic properties of the influenza polypeptides themselves.
已构建出一套完整的表达流感病毒各多肽的重组痘苗病毒。PB1、PB2、PA、HA、NP、M1和NS1基因源自甲型流感病毒A/PR/8/34，NA源自甲型流感病毒A/Cam/46，M2和NS2基因源自甲型流感病毒A/Udorn/72。感染这些重组病毒的细胞合成的流感多肽，能用特异性抗血清沉淀，且其电泳迁移率与相应的流感病毒多肽相似。间接免疫荧光研究表明，HA、NA和MS2蛋白迁移至细胞表面；PB2、PB1、PA、NP和NS1蛋白迁移至细胞核；M1和NS2分布于整个细胞，不过NS2优先在细胞核中积累。这些转运过程独立于其他流感多肽发生，因此归因于流感多肽自身的内在特性。
