Department of General Surgery, Caoxian People's Hospital, Heze, Shandong 274400, China.
Department of Gastroenterology, Caoxian People's Hospital, Heze, Shandong 274400, China.
Cancer Biomark. 2021;30(2):167-177. doi: 10.3233/CBM-201754.
Circular RNA (circRNA) is a class of non-coding RNA that is vital for regulating gene expression and biological functions. Mounting studies demonstrate that circRNA is crucial for human cancer development. However, the role of circ_0000039 in gastric cancer (GC) remains uncertain.
Normal human gastric tissues and GC tissue samples were collected, and quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect the expression levels of circ_0000039, miR-1292-5p, and DEK. GC cell lines with overexpression and low expression of circ_0000039 were constructed. Cell counting kit-8 (CCK-8), scratch healing and Transwell experiments were used to assess the function of circ_0000039 on the proliferation, migration and invasion of GC cells. Bioinformatics analysis and dual-luciferase reporter assays were employed to detect the targeting relationship between circ_0000039 and miR-1292-5p.
Circ_0000039 expression was up-regulated in GC tissues and cell lines, and it was significantly related with poor differentiation of tumor tissues. In addition, circ_0000039 overexpression enhanced the proliferation, migration and invasion of GC cells, while circ_0000039 depletion inhibited these malignant biological behaviors. In terms of mechanism, it was found that circ_0000039 promoted the proliferation and progression of GC cells by adsorbing miR-1292-5p and up-regulating the expression of DEK.
Circ_0000039 is a new oncogenic circRNA in GC, which regulates the miR-1292-5p/DEK axis to modulate the malignant biological behaviors of GC.
环状 RNA（circRNA）是一类对基因表达和生物功能的调控至关重要的非编码 RNA。越来越多的研究表明，circRNA 对人类癌症的发生发展起着关键作用。然而，circ_0000039 在胃癌（GC）中的作用尚不清楚。
收集正常人类胃组织和 GC 组织样本，采用实时定量聚合酶链反应（qRT-PCR）检测 circ_0000039、miR-1292-5p 和 DEK 的表达水平。构建 circ_0000039 过表达和低表达的 GC 细胞系。细胞计数试剂盒-8（CCK-8）、划痕愈合和 Transwell 实验用于评估 circ_0000039 对 GC 细胞增殖、迁移和侵袭的功能。生物信息学分析和双荧光素酶报告基因实验用于检测 circ_0000039 与 miR-1292-5p 之间的靶向关系。
circ_0000039 在 GC 组织和细胞系中表达上调，与肿瘤组织的低分化显著相关。此外，circ_0000039 过表达增强了 GC 细胞的增殖、迁移和侵袭能力，而 circ_0000039 耗竭则抑制了这些恶性生物学行为。就机制而言，研究发现 circ_0000039 通过吸附 miR-1292-5p 和上调 DEK 的表达来促进 GC 细胞的增殖和进展。
circ_0000039 是 GC 中的一种新的致癌 circRNA，通过调节 miR-1292-5p/DEK 轴来调节 GC 的恶性生物学行为。
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