Department of Geriatrics, the Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
Department of Gastroenterology, the Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
Cell Cycle. 2022 Oct;21(20):2145-2164. doi: 10.1080/15384101.2022.2087280. Epub 2022 Jun 16.
circFCHO2 has been revealed to be overexpressed in gastric cancer (GC) patients. This article identified the function of circFCHO2 on GC progression. The expression of circFCHO2, miR-194-5p and JAK1 in 30 GC patients and cells was monitored by quantitative reverse transcription-polymerase chain reaction. circFCHO2 localization in GC cells was monitored by RNA fluorescence in situ hybridization. Cell counting kit-8 assay, 5-ethynyl-2-deoxyuridine staining, transwell experiment, tube formation and sphere formation experiments were applied to detect GC cell proliferation, invasion, angiogenesis and cancer stem cell characteristics. Dual-luciferase reporter gene assay, RNA pull down assay and RNA immunoprecipitation experiment were utilized to research the binding between two genes. In vivo tumorigenesis and lung metastasis were studied using nude mice. Immunohistochemistry and hematoxylin-eosin staining were conducted. Protein expression was assessed by Western blot. Serum exosomes of GC patients and healthy participants were isolated. circFCHO2 up-modulation in GC patients was related to poor outcome. circFCHO2 was located in the cytoplasm of GC cells. circFCHO2 silencing weakened the proliferation, invasion, angiogenesis and stem cell characteristics of GC cells. miR-194-5p knockdown counteracted this effect. circFCHO2 activated the JAK1/STAT3 pathway by sponging miR-194-5p. miR-194-5p overexpression attenuated the malignant phenotypes of GC cells. JAK1 overexpression abrogated this effect. circFCHO2 silencing weakened GC cells growth and lung metastasis in vivo. circFCHO2 was up-modulated in serum exosomes of GC patients. circFCHO2 was an oncogene in GC by activating the JAK1/STAT3 pathway via sponging miR-194-5p. circFCHO2 might be a novel target and diagnostic marker for GC.
circFCHO2 在胃癌（GC）患者中被证实过表达。本文旨在确定 circFCHO2 对 GC 进展的作用。通过定量逆转录-聚合酶链反应监测 30 名 GC 患者和细胞中 circFCHO2、miR-194-5p 和 JAK1 的表达。通过 RNA 荧光原位杂交监测 GC 细胞中 circFCHO2 的定位。细胞计数试剂盒-8 检测、5-乙炔基-2-脱氧尿苷染色、transwell 实验、管形成和球体形成实验用于检测 GC 细胞的增殖、侵袭、血管生成和癌症干细胞特征。双荧光素酶报告基因检测、RNA 下拉检测和 RNA 免疫沉淀实验用于研究两个基因的结合。利用裸鼠研究体内肿瘤发生和肺转移。进行免疫组织化学和苏木精-伊红染色。通过 Western blot 评估蛋白表达。分离 GC 患者和健康参与者的血清外泌体。GC 患者 circFCHO2 的上调与不良预后相关。circFCHO2 位于 GC 细胞的细胞质中。circFCHO2 沉默削弱了 GC 细胞的增殖、侵袭、血管生成和干细胞特征。miR-194-5p 敲低可拮抗该作用。circFCHO2 通过海绵吸附 miR-194-5p 激活 JAK1/STAT3 通路。miR-194-5p 过表达可减轻 GC 细胞的恶性表型。JAK1 过表达可消除这种作用。circFCHO2 沉默可减弱 GC 细胞体内生长和肺转移。GC 患者血清外泌体中 circFCHO2 上调。circFCHO2 通过海绵吸附 miR-194-5p 激活 JAK1/STAT3 通路，是 GC 的致癌基因。circFCHO2 可能是 GC 的一个新靶点和诊断标志物。
