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突尼斯产 Turra 根的两种有机提取物的酚类成分(HPLC-UV)分析及生物活性。

Phenolic profile (HPLC-UV) analysis and biological activities of two organic extracts from Turra roots growing in Tunisia.

作者信息

Khedher Olfa, Rigane Ghayth, Riguene Hajer, Ben Salem Ridha, Moussaoui Younes

机构信息

Organic Chemistry Laboratory LR17ES08, Sciences Faculty of Sfax, Chemistry Department, University of Sfax, Sfax, Tunisia.

Physics and Chemistry Department, Sciences and Technology Faculty of Sidi Bouzid, University of Kairouan, Sidi Bouzid, Tunisia.

出版信息

Nat Prod Res. 2021 Dec;35(24):5786-5793. doi: 10.1080/14786419.2020.1837812. Epub 2020 Oct 27.

DOI:10.1080/14786419.2020.1837812
PMID:33107347
Abstract

Turra subsp. roots was investigated, after extraction with two different solvents: ethanol and ethyl acetate, for its phenolic profile as well as biological activities. Turra subsp. roots ethanolic extract was characterized by the highest content of total polyphenols (19.3 mg GAE/100 g of DW). In addition, the phenolic profiles of the two studied extracts were analysed using HPLC-UV, where -coumaric (8.59 mg/kg of DW) and cinnamic acids (4.68 mg/kg of DW) were detected as the most abundant phenolic acids, and kaempferol was the predominant flavonoids (30.37 mg/kg of DM) followed by quercetin and rutin in the ethanolic extract. While, only -coumaric acid, epicatechin, kaempferol and quercetin were identified and quantified in ethyl acetate roots extract. All tested extracts exhibited an antimicrobial effect against Gram-positive and Gram-negative bacteria. These findings indicate that Turra subsp. roots can be regarded as a new source of edible oil having health benefits and nutritional properties.

摘要

Turra subsp. 的根经过两种不同溶剂(乙醇和乙酸乙酯)提取后,对其酚类成分和生物活性进行了研究。Turra subsp. 的乙醇根提取物总多酚含量最高(19.3 mg GAE/100 g DW)。此外,采用 HPLC-UV 分析了两种研究提取物的酚类成分,其中 -香豆酸(8.59 mg/kg DW)和肉桂酸(4.68 mg/kg DW)被检测为最丰富的酚酸,而山奈酚(kaempferol)则是乙醇根提取物中含量最丰富的类黄酮(30.37 mg/kg DM),其次是槲皮素和芦丁。而在乙酸乙酯根提取物中仅鉴定和定量了 -香豆酸、表儿茶素、山奈酚和槲皮素。所有测试的提取物都表现出对革兰氏阳性菌和革兰氏阴性菌的抗菌作用。这些发现表明,Turra subsp. 的根可以被视为一种具有健康益处和营养特性的新型食用油来源。

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