探讨肝癌中高表达的长非编码 RNA（HULC）在乳腺癌中的作用：一项临床研究。
Study of long non-coding RNA highly upregulated in liver cancer (HULC) in breast cancer: A clinical & investigation.
机构信息
Department of Animal Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of Pathology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Indian J Med Res. 2020 Sep;152(3):244-253. doi: 10.4103/ijmr.IJMR_1823_18.
BACKGROUND & OBJECTIVES: Breast cancer remains the most common malignancy among women worldwide. Long non-coding RNAs (lncRNAs) have been shown to play critical roles in tumour initiation and progression. This study was aimed to evaluate the potential role of lncRNA highly upregulated in liver cancer (HULC) in breast cancer.
METHODS
The expression of HULC was evaluated in breast cancer patients and cell lines using real-time quantitative reverse transcription polymerase chain reaction. Small interfering RNA-based knockdown was also employed to study the potential role of HULC in breast cancer cell lines including ZR-75-1, MCF7 and MDA-MB-231.
RESULTS
HULC was significantly upregulated in tumour tissues compared to non-tumoural margins (P <0.001). The receiver operating characteristic (ROC) curve analysis demonstrated the biomarker potential of HULC (ROC=0.78, P <0.001). The HULC knockdown induced apoptosis and suppressed cellular migration in breast cancer cell lines.
INTERPRETATION & CONCLUSIONS: Our results indicated that HULC was upregulated in breast cancer and might play a role in tumourigenesis. The HULC may have a potential to be exploited as a new biomarker and therapeutic target in breast cancer.
背景与目的
乳腺癌仍然是全球女性最常见的恶性肿瘤。长链非编码 RNA（lncRNA）已被证明在肿瘤的发生和发展中发挥关键作用。本研究旨在评估肝癌中高表达的 lncRNA（HULC）在乳腺癌中的潜在作用。
方法
采用实时定量逆转录聚合酶链反应检测乳腺癌患者和细胞系中 HULC 的表达。还采用小干扰 RNA 敲低技术研究 HULC 在乳腺癌细胞系 ZR-75-1、MCF7 和 MDA-MB-231 中的潜在作用。
结果
与非肿瘤边缘相比，肿瘤组织中 HULC 显著上调（P<0.001）。受试者工作特征（ROC）曲线分析显示 HULC 具有作为生物标志物的潜力（ROC=0.78，P<0.001）。HULC 敲低可诱导乳腺癌细胞系中的细胞凋亡并抑制细胞迁移。
结论与解释
我们的结果表明 HULC 在乳腺癌中上调，可能在肿瘤发生中发挥作用。HULC 可能有潜力成为乳腺癌新的生物标志物和治疗靶点。
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