Yan Hong, Tian Rui, Zhang Min, Wu Jing, Ding Min, He Jie
Department of Pathology, Anhui Provincial Hospital affiliated to Anhui Medical University and Anhui Provincial Cancer Hospital, Hefei, Anhui, People's Republic of China.
Onco Targets Ther. 2016 Dec 21;10:113-120. doi: 10.2147/OTT.S124614. eCollection 2017.
Emerging studies show that long noncoding RNAs (lncRNAs) have important roles in carcinogenesis. This study investigated the role of lncRNA highly upregulated in liver cancer (HULC) expression in glioma and its clinical significance in glioma patients.
HULC expression was detected in glioma tissues and cell lines by using real-time quantitative reverse transcription polymerase chain reactions. Association between HULC levels and clinicopathological factors and patients prognosis was also analyzed. Expression of HULC was restored and knocked down in glioma cell line U87 by using HULC cDNA and siRNA, respectively. CCK-8 and colony formation assays were used to investigate the role of HULC in the regulation of proliferation of glioma cells.
HULC was highly expressed in glioma tissues, being closely related to age and grade of glioma. Univariate survival analysis demonstrated that high HULC levels were significantly associated with overall survival (OS) (hazard ratio [HR], 0.422; 95% confidence interval [CI], 0.220-0.806; =0.009), and it remained an independent predictor for OS (HR, 0.340; 95% CI, 0.175-0.659; =0.001) in multivariate Cox regression analysis. Functionally, forced expression of HULC results in increased cell proliferation and colony formation of U87 glioma cell line, whereas knockdown of HULC expression reduced these oncogenic properties of glioma cells.
These findings suggest that HULC may play an important role in glioma progression and will be further evaluated as a biomarker for predicting the survival of glioma patients.
新出现的研究表明，长链非编码RNA（lncRNA）在肿瘤发生中起重要作用。本研究探讨了肝癌中高度上调的lncRNA（HULC）在胶质瘤中的作用及其在胶质瘤患者中的临床意义。
采用实时定量逆转录聚合酶链反应检测胶质瘤组织和细胞系中HULC的表达。分析HULC水平与临床病理因素及患者预后的相关性。分别使用HULC cDNA和小干扰RNA（siRNA）在胶质瘤细胞系U87中恢复和敲低HULC的表达。采用细胞计数试剂盒-8（CCK-8）和集落形成试验研究HULC在调节胶质瘤细胞增殖中的作用。
HULC在胶质瘤组织中高表达，与胶质瘤的年龄和分级密切相关。单因素生存分析表明，HULC高水平与总生存期（OS）显著相关（风险比[HR]，0.422；95%置信区间[CI]，0.220-0.806；P=0.009），在多因素Cox回归分析中，它仍然是OS的独立预测因子（HR，0.340；95%CI，0.175-0.659；P=0.001）。在功能上，强制表达HULC导致U87胶质瘤细胞系的细胞增殖和集落形成增加，而敲低HULC表达则降低了胶质瘤细胞的这些致癌特性。
这些发现表明，HULC可能在胶质瘤进展中起重要作用，并将作为预测胶质瘤患者生存的生物标志物进行进一步评估。
