Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, 29 The Thirteenth Road, Tianjin Economy and Technology Development Area, Tianjin 300457, P.R. China.
Medical college, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin 300350, P.R. China.
J Agric Food Chem. 2020 Nov 11;68(45):12738-12748. doi: 10.1021/acs.jafc.0c05308. Epub 2020 Oct 27.
Evaluation of the nutrition and determination of phosphoproteins is of great importance in different foods as aberrant phosphorylation changes many biological processes and can relate to health conditions. In this study, an ultrafast (5 min) and sensitive electrochemiluminescence (ECL) sensor was innovatively fabricated for the determination of phosphoproteins in foods on the basis of fluorescent probe NH-TiO/upconversion nanomaterials (UCNPs). Impressively, the ECL intensity of NH-TiO/UCNPs-rGO/GCE was remarkably enhanced by 29 times. Furthermore, the photoactive NH-TiO layer provided not only specific selectivity but also a large surface area as well as an unprecedented photocatalytic activity for the NH-TiO/UCNPs-rGO/GCE ECL sensor (TIECLS), which could serve as an identification element for trace phosphoproteins. Under optimal conditions, the TIECLS achieved a relatively low detection limit of 9.2 × 10 mg/mL (/ = 3). Practical application of this TIECLS was carried out in different food samples with satisfying results, which were validated by laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS).
评估不同食物中的营养成分和确定磷酸化蛋白具有重要意义，因为异常磷酸化会改变许多生物过程，并且可能与健康状况有关。在这项研究中，基于荧光探针 NH-TiO/上转换纳米材料（UCNPs），创新地构建了一种超快速（5 分钟）和灵敏的电致化学发光（ECL）传感器，用于测定食物中的磷酸化蛋白。令人印象深刻的是，NH-TiO/UCNPs-rGO/GCE 的 ECL 强度显著增强了 29 倍。此外，光活性 NH-TiO 层不仅提供了特异性选择性，而且还提供了大的表面积和前所未有的 NH-TiO/UCNPs-rGO/GCE ECL 传感器（TIECLS）的光催化活性，可作为痕量磷酸化蛋白的识别元件。在最佳条件下，TIECLS 实现了相对较低的检测限 9.2×10 mg/mL（/ = 3）。通过激光解吸电离飞行时间质谱（MALDI-TOF MS）对不同食品样品进行了实际应用，结果令人满意。
