Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science & Technology, 29 The Thirteenth Road, Tianjin Economy and Technology Development Area, Tianjin 300457, PR China.
Medical College, Nankai University, No.38 Tongyan Road, Jinnan District, Tianjin 300350, PR China.
Food Chem. 2021 May 15;344:128656. doi: 10.1016/j.foodchem.2020.128656. Epub 2020 Nov 18.
A novel quartz crystal microbalance (QCM) sensor for trace-phosphoprotein ultrafast detection was constructed based on the bridge interactions between the NH-TiO sites enriched on Au-electrode and phosphate groups. Herein, 11-mercaptoundecanoic acid (MUA) modified by Au-S bond acted as carrier for immobilizing NH-TiO. Functionalized NH-TiO to absorb phosphoproteins. Under the optimal conditions, the proposed sensor showed a linear frequency shift to the concentration of α-casein ranging from 1.0 × 10 to 1.0 mg mL with a low detection limit of 5.3 × 10 mg mL (S/N = 3), and the limit of quantitation was 0.001 mg mL. Compared with traditional Ti-IMAC/MOAC-system, the analysis process of NH-TiO/MUA/AuE-QCM sensor was simpler and faster which could complete within 5 min. Additionally, the constructed biosensor was successfully used for the non-fat milk and chicken egg white. This proposed sensor presents a great prospective strategy for the evaluation of the nutrition in different foods.
基于金电极上富集的 NH-TiO 位点与磷酸基团之间的桥接相互作用，构建了用于痕量磷蛋白超快检测的新型石英晶体微天平（QCM）传感器。在此，通过 Au-S 键修饰的 11-巯基十一酸（MUA）用作固定 NH-TiO 的载体。功能化的 NH-TiO 用于吸收磷蛋白。在最佳条件下，该传感器对 α-酪蛋白浓度的线性频率偏移范围为 1.0×10 至 1.0 mg mL，检测下限低至 5.3×10 mg mL（S/N=3），定量下限为 0.001 mg mL。与传统的 Ti-IMAC/MOAC 系统相比，NH-TiO/MUA/AuE-QCM 传感器的分析过程更简单、更快，可在 5 min 内完成。此外，所构建的生物传感器已成功用于非脂牛奶和鸡蛋白。该传感器为不同食品中营养成分的评估提供了一种很有前景的策略。
