Department of Otolaryngology-HNS, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Fudan University School of Medicine, Shanghai, People's Republic of China.
Department of Pudong Hospital, Fudan University School of Medicine, Shanghai, People's Republic of China.
Laryngoscope. 2021 Apr;131(4):E1249-E1255. doi: 10.1002/lary.29196. Epub 2020 Oct 27.
OBJECTIVES/HYPOTHESIS: Tumor-infiltrating lymphocytes (TILs) has been shown to be associated with the prognosis of many tumors, yet few studies have investigated their roles in laryngeal squamous cell carcinoma (LSCC). We aim to investigate the prognostic values of tumor-infiltrating CD3 /CD4 /CD8 /Foxp3 T-cells and neutrophils in LSCC patients that received total or partial laryngectomy.
Retrospective case series of LSCC patients who underwent total or partial laryngectomy from 2013 to 2014 at Eye, Ear, Nose, and Throat Hospital of Fudan University.
In our study, 41 tumor tissues from patients with LSCC were retrospectively assessed using immunohistochemistry for CD3 /CD4 /CD8 /Foxp3 T-cells and CD66b neutrophils. Overall survival (OS) and disease-free survival (DFS) were recorded using Kaplan-Meier methods.
Generally, patients with high density of TILs (CD3, CD4, CD8) showed improved OS or DFS. Specifically, high density of CD3 TILs were associated with better OS, yet poorer OS and DFS for CD66b neutrophils. Patients with an Immunoscore of 0-1 experienced the worst OS and DFS, compared with Immunoscore 2-4 (P = .0111 for OS, P = .0391 for DFS). In Cox proportional hazards analysis adjusted for N stage and T stage, only stroma CD66b neutrophils densities were able to predict OS, with odds ratios of 4.819 (95% confidence interval [CI] 1.149-20.206; P = .032*), and DFS 2.888 (95% CI 1.043-7.997; P = .041*).
The density of TILs and CD66b neutrophils may help predict the prognosis of patients with LSCC after surgery.
3 Laryngoscope, 131:E1249-E1255, 2021.
目的/假设：肿瘤浸润淋巴细胞（TILs）已被证明与许多肿瘤的预后相关，但很少有研究探讨其在喉鳞状细胞癌（LSCC）中的作用。我们旨在研究接受全喉或部分喉切除术的 LSCC 患者的肿瘤浸润 CD3/CD4/CD8/Foxp3 T 细胞和中性粒细胞的预后价值。
回顾性病例系列研究，纳入 2013 年至 2014 年在复旦大学眼耳鼻喉科医院接受全喉或部分喉切除术的 LSCC 患者。
我们使用免疫组织化学方法检测了 41 例 LSCC 肿瘤组织中的 CD3/CD4/CD8/Foxp3 T 细胞和 CD66b 中性粒细胞。使用 Kaplan-Meier 方法记录总生存（OS）和无病生存（DFS）。
一般来说，TILs（CD3、CD4、CD8）密度高的患者 OS 或 DFS 改善。具体而言，CD3 TILs 密度高与 OS 较好相关，而 CD66b 中性粒细胞密度高与 OS 和 DFS 较差相关。免疫评分 0-1 的患者 OS 和 DFS 最差，与免疫评分 2-4 的患者相比（OS 的 P =.0111，DFS 的 P =.0391）。在调整 N 分期和 T 分期的 Cox 比例风险分析中，只有基质 CD66b 中性粒细胞密度能够预测 OS，优势比为 4.819（95%置信区间[CI]为 1.149-20.206；P =.032*），DFS 为 2.888（95%CI 为 1.043-7.997；P =.041*）。
TILs 和 CD66b 中性粒细胞的密度可能有助于预测 LSCC 患者手术后的预后。
3 级喉镜，131：E1249-E1255，2021。
