D'Mello A, Yotis W W
Department of Microbiology, Stritch School of Medicine, Loyola University, Maywood, Illinois 60153.
Appl Environ Microbiol. 1987 Aug;53(8):1944-6. doi: 10.1128/aem.53.8.1944-1946.1987.
Sodium deoxycholate is used in a number of bacteriological media for the isolation and classification of gram-negative bacteria from food and the environment. Initial experiments to study the effect of deoxycholate on the growth parameters of Escherichia coli showed an increase in the lag time constant and generation time and a decrease in the growth rate constant and total cell yield of this microorganism. Cell fractionation studies indicated that sodium deoxycholate at levels used in bacteriological media interferes with the incorporation of [U-14C]glucose into the cold-trichloroacetic acid-soluble, ethanol-soluble, and trypsin-soluble cellular fractions of E. coli. Finally, sodium deoxycholate interfered with the flagellation and motility of Proteus mirabilis and E. coli. It would appear then that further improvement of the deoxycholate medium may be in order.
脱氧胆酸钠用于多种细菌学培养基中，用于从食品和环境中分离和分类革兰氏阴性菌。最初研究脱氧胆酸钠对大肠杆菌生长参数影响的实验表明，该微生物的延迟时间常数和世代时间增加，生长速率常数和总细胞产量降低。细胞分级分离研究表明，细菌学培养基中使用的脱氧胆酸钠水平会干扰[U-14C]葡萄糖掺入大肠杆菌的冷三氯乙酸可溶性、乙醇可溶性和胰蛋白酶可溶性细胞组分中。最后，脱氧胆酸钠干扰了奇异变形杆菌和大肠杆菌的鞭毛形成和运动。因此，脱氧胆酸钠培养基可能需要进一步改进。
