Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, India.
Prep Biochem Biotechnol. 2021;51(6):550-561. doi: 10.1080/10826068.2020.1836655. Epub 2020 Oct 28.
Conventional cotton scouring in the textile industry using alkali results in huge environmental impact which can be overcome by using enzymes. Pectinase along with cutinase gives enhanced bioscouring results. Cutin was extracted from tomato peels and was used as substrate in the microbial media. The strain isolated from tomato peel was identified as by 16S rDNA sequencing. The cutinase production was optimized by Placket-Burman and Response Surface Methodology (RSM) and the maximum production of 82.75 U/mL obtained at sucrose 6.68% (w/v), gelatin 2.74 g/L at a temperature of 35.93 °C. Cutinase was purified by ammonium sulfate precipitation, hydrophobic interaction chromatography and ion exchange chromatography with a recovery of 25.6% and specific activity of 38030 U/mg. The confirmation test for the purity of cutinase was analyzed by RP-HPLC. The molecular mass of cutinase was determined as 28.9 kDa by SDS-PAGE technique. Scanning electron microscopic analysis showed a rough and open primary wall surface on the cutinase bioscoured fabric which confirmed its activity on cutin present in the cotton fabric. Additionally, the cutinase-bioscoured samples showed better absorbency than the untreated samples. Therefore, enzymatic scouring increases wetting capacity of scoured cotton and also helps to reduce environmental pollution.
在纺织工业中，传统的棉织物精练使用碱会对环境造成巨大影响，而使用酶可以克服这一问题。果胶酶和角质酶的联合使用可以带来更好的生物精练效果。从番茄皮中提取角质，并将其用作微生物培养基中的底物。从番茄皮中分离出的菌株通过 16S rDNA 测序被鉴定为。通过 Placket-Burman 和响应面法（RSM）对角质酶的生产进行了优化，在蔗糖 6.68%（w/v）、明胶 2.74g/L 的条件下，温度为 35.93°C 时，获得了最大的 82.75U/mL 的产量。通过硫酸铵沉淀、疏水相互作用色谱和离子交换色谱对角质酶进行了纯化，回收率为 25.6%，比活为 38030U/mg。通过反相高效液相色谱（RP-HPLC）对角质酶的纯度进行了确认试验。通过 SDS-PAGE 技术确定角质酶的分子量为 28.9kDa。扫描电子显微镜分析显示，经过角质酶生物精练的织物的初生壁表面粗糙且开放，这证实了其对棉织物中存在的角质的活性。此外，经过角质酶生物精练的样品比未经处理的样品具有更好的吸水性。因此，酶精练可以提高精练棉的润湿性，同时有助于减少环境污染。
