Aladdin Nor-Ashila, Husain Khairana, Jalil Juriyati, Sabandar Carla Wulandari, Jamal Jamia Azdina
Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Department of Pharmacy, Faculty of Science and Technology, Universitas Sembilanbelas November Kolaka, Kolaka, Indonesia.
BMC Complement Med Ther. 2020 Oct 27;20(1):324. doi: 10.1186/s12906-020-03119-8.
In traditional Malay medicine, Marantodes pumilum (Blume) Kuntze (family Primulaceae) is commonly used by women to treat parturition, flatulence, dysentery, dysmenorrhea, gonorrhea, and bone diseases. Preliminary screening of some Primulaceae species showed that they possess xanthine oxidase inhibitory activity. Thus, this study aimed to investigate the xanthine oxidase inhibitory activity of three varieties of M. pumilum and their phytochemical compounds.
Dichloromethane, methanol, and water extracts of the leaves and roots of M. pumilum var. alata, M. pumilum var. pumila, and M. pumilum var. lanceolata were tested using an in vitro xanthine oxidase inhibitory assay. Bioassay-guided fractionation and isolation were carried out on the most active extract using chromatographic techniques. The structures of the isolated compounds were determined using spectroscopic techniques.
The most active dichloromethane extract of M. pumilum var. pumila leaves (IC = 161.6 μg/mL) yielded one new compound, 3,7-dihydroxy-5-methoxy-4,8-dimethyl-isocoumarin (1), and five known compounds, viz. ardisiaquinone A (2), maesanin (3), stigmasterol (4), tetracosane (5), and margaric acid (6). The new compound was found to be the most active xanthine oxidase inhibitor with an IC value of 0.66 ± 0.01 μg/mL, which was not significantly different (p > 0.05) from that of the positive control, allopurinol (IC = 0.24 ± 0.00 μg/mL).
This study suggests that the new compound 3,7-dihydroxy-5-methoxy-4,8-dimethyl-isocoumarin (1), which was isolated from the dichloromethane extract of M. pumilum var. pumila leaves, could be a potential xanthine oxidase inhibitor.
在传统马来医学中，矮小报春（报春花科）被女性广泛用于治疗分娩、肠胃胀气、痢疾、痛经、淋病和骨骼疾病。对一些报春花科物种的初步筛选表明，它们具有黄嘌呤氧化酶抑制活性。因此，本研究旨在调查三种矮小报春及其植物化学化合物的黄嘌呤氧化酶抑制活性。
使用体外黄嘌呤氧化酶抑制试验测试了矮小报春翼叶变种、矮小报春原变种和矮小报春披针叶变种的叶和根的二氯甲烷、甲醇和水提取物。使用色谱技术对活性最强的提取物进行生物测定导向的分级分离和分离。使用光谱技术确定分离化合物的结构。
矮小报春原变种叶的活性最强的二氯甲烷提取物（IC = 161.6 μg/mL）产生了一种新化合物，3,7-二羟基-5-甲氧基-4,8-二甲基异香豆素（1），以及五种已知化合物，即紫金牛醌A（2）、迈桑宁（3）、豆甾醇（4）、二十四烷（5）和十七烷酸（6）。发现该新化合物是活性最强的黄嘌呤氧化酶抑制剂，IC值为0.66±0.01 μg/mL，与阳性对照别嘌呤醇（IC = 0.24±0.00 μg/mL）相比无显著差异（p＞0.05）。
本研究表明，从矮小报春原变种叶的二氯甲烷提取物中分离出的新化合物3,7-二羟基-5-甲氧基-4,8-二甲基异香豆素（1）可能是一种潜在的黄嘌呤氧化酶抑制剂。
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