SNHG17 通过海绵吸附 miR-485-5p 上调 WLS 的表达来加速肺腺癌的进展。
SNHG17 upregulates WLS expression to accelerate lung adenocarcinoma progression by sponging miR-485-5p.
机构信息
Department of Respiration, West China Hospital, Sichuan University, Chengdu, 610000, China; Department of Respiratory and Critical Care Medicine, Chengdu First People's Hospital, Chengdu, 610000, China.
Department of Respiratory and Critical Care Medicine, Chengdu First People's Hospital, Chengdu, 610000, China.
出版信息
Biochem Biophys Res Commun. 2020 Dec 17;533(4):1435-1441. doi: 10.1016/j.bbrc.2020.09.130. Epub 2020 Oct 24.
BACKGROUND
Long non-coding RNAs (lncRNAs) have been uncovered to be essential regulators in the biological processes of human cancers, including lung adenocarcinoma (LUAD). Recently, small nucleolar RNA host gene 17 (SNHG17) has been identified as one novel oncogenic lncRNA in gastric cancer. However, it remains unclear whether SNHG7 exert functions in LUAD progression.
METHODS
The expression levels of SNHG17, miR-485-5p and Wnt ligand secretion mediator (WLS) in LUAD cells was evaluated by RT-qPCR. The effect of SNHG7 silencing on LUAD cell proliferation was assessed by colony formation and EdU assays. The apoptosis of LUAD cells was measured by flow cytometry analysis. Transwell assays were applied to detect cell migration and invasion. The relationship between SNHG17 and miR-485-5p was validated by RIP, RNA pull down and luciferase reporter assays.
RESULTS
SNHG17 and WLS were up-regulated in LUAD cell lines. Down-regulation of SNHG17 curbed LUAD cell proliferation, migration and invasion but facilitated apoptosis. SNHG17 acted as miR-485-5p sponge to upregulate WLS expression.
CONCLUSION
SNHG17 triggers the progression of LUAD via sponging miR-485-5p to upregulate WLS expression.
背景
长链非编码 RNA（lncRNA）已被发现是人类癌症生物学过程中的重要调控因子，包括肺腺癌（LUAD）。最近，小核仁 RNA 宿主基因 17（SNHG17）已被确定为胃癌中的一种新型致癌 lncRNA。然而，SNHG7 是否在 LUAD 进展中发挥作用尚不清楚。
方法
通过 RT-qPCR 评估 LUAD 细胞中 SNHG17、miR-485-5p 和 Wnt 配体分泌介质（WLS）的表达水平。通过集落形成和 EdU 测定评估 SNHG7 沉默对 LUAD 细胞增殖的影响。通过流式细胞术分析检测 LUAD 细胞的凋亡。通过 Transwell 测定检测细胞迁移和侵袭。通过 RIP、RNA 下拉和荧光素酶报告基因测定验证 SNHG17 和 miR-485-5p 之间的关系。
结果
SNHG17 和 WLS 在 LUAD 细胞系中上调。SNHG17 的下调抑制 LUAD 细胞增殖、迁移和侵袭，但促进凋亡。SNHG17 作为 miR-485-5p 的海绵体上调 WLS 表达。
结论
SNHG17 通过海绵 miR-485-5p 上调 WLS 表达引发 LUAD 的进展。
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